A method for determination of fruit-derived ascorbic, tartaric, oxalic and malic acids, and its application to the study of ascorbic acid catabolism in grapevines

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dc.contributor.authorMelino, V.
dc.contributor.authorSoole, K.
dc.contributor.authorFord, C.
dc.date.issued2009
dc.description.abstractBackground and Aims:  The majority of the acidity of a grapevine (Vitis vinifera L.) berry is a result of the accumulation of l-tartaric (TA) and l-malic acids (MA). TA is synthesised from l-ascorbic acid (Asc, vitamin C), the metabolism of which is poorly characterised in grapevines. In a distinct pathway, oxalic acid (OA) is also formed from Asc degradation. The aim of this study was to develop a single method whereby the distribution of Asc and its catabolites from fruit and vegetative sources could be determined. Methods and Results:  Effective recoveries of total Asc, TA, OA and MA were achieved with this extraction method, while chromatographic separation was accomplished with reversed-phase high-performance liquid chromatography (RP-HPLC). These results demonstrate that Asc and its catabolites TA and OA rapidly accumulate in immature berries, and that the Asc to dehydroascorbate ratio increases with berry maturity. Conclusions:  A method for the simultaneous analysis of Asc, TA, OA and MA in fruits is provided; moreover, we have demonstrated its use to study their distribution in fruits, rachis, leaves and roots. Significance of the Study:  This method enables accurate monitoring of the accumulation of Asc, permitting further research towards understanding acid metabolism during berry ripening.
dc.description.statementofresponsibilityV.J. Melino, K.L. Soole and C.M. Ford
dc.identifier.citationAustralian Journal of Grape and Wine Research, 2009; 15(3):293-302
dc.identifier.doi10.1111/j.1755-0238.2009.00062.x
dc.identifier.issn1322-7130
dc.identifier.issn1755-0238
dc.identifier.orcidMelino, V. [0000-0003-2742-5079]
dc.identifier.orcidFord, C. [0000-0003-1617-2977]
dc.identifier.urihttp://hdl.handle.net/2440/60440
dc.language.isoen
dc.publisherAustralian Society of Viticulture and Oenology
dc.rights© 2009 Australian Society of Viticulture and Oenology Inc.
dc.source.urihttps://doi.org/10.1111/j.1755-0238.2009.00062.x
dc.subjectascorbate
dc.subjectberry development
dc.subjectcatabolism
dc.subjectmalic acid
dc.subjectoxalic acid
dc.subjecttartaric acid
dc.titleA method for determination of fruit-derived ascorbic, tartaric, oxalic and malic acids, and its application to the study of ascorbic acid catabolism in grapevines
dc.typeJournal article
pubs.publication-statusPublished

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