A 2.5-Mb contig constructed from Angus Longhorn and horned Hereford DNA spanning the polled interval on bovine chromosome 1
Date
2006
Authors
Wunderlich, K.
Abbey, C.
Clayton, D.
Song, Y.
Schein, J.
Georges, M.
Coppieters, W.
Adelson, D.
Taylor, J.
Davis, S.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
Animal Genetics, 2006; 37(6):592-594
Statement of Responsibility
K. R. Wunderlich, C. A. Abbey, D. R. Clayton, Y. Song, J. E. Schein, M. Georges, W. Coppieters, D. L. Adelson, J. F. Taylor, S. L. Davis and C. A. Gil
Conference Name
Abstract
The polled locus has been mapped by genetic linkage analysis to the proximal region of bovine chromosome 1. As an intermediate step in our efforts to identify the polled locus and the underlying causative mutation for the polled phenotype, we have constructed a BACbased physical map of the interval containing the polled locus. Clones containing genes and markers in the critical interval were isolated from the TAMBT (constructed from Angus and Longhorn genomic DNA) and CHORI-240 (constructed from horned Hereford genomic DNA) BAC libraries and ordered based on fingerprinting and the presence or absence of 80 STS markers. A single contig spanning 2.5 Mb was assembled. Comparison of the physical order of STSs to the corresponding region of human chromosome 21 revealed the same order of genes within the polled critical interval. This contig of overlapping BAC clones from horned and polled breeds is a useful resource for SNP discovery and characterization of positional candidate genes.
School/Discipline
Dissertation Note
Provenance
Description
The definitive version is available at www.blackwell-synergy.com