Assessment of probiotic properties of lactic acid bacteria isolated from Indonesian naturally fermented milk
Date
2017
Authors
Jatmiko, Y.
Howarth, G.
Barton, M.
Editors
Salunkhe, C.
Kikuchi, A.
Govinda, B.
Jatmiko, Y.
Siswanto, D.
Kikuchi, A.
Govinda, B.
Jatmiko, Y.
Siswanto, D.
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Conference paper
Citation
AIP Conference Proceedings, 2017 / Salunkhe, C., Kikuchi, A., Govinda, B., Jatmiko, Y., Siswanto, D. (ed./s), vol.1908, iss.050008, pp.050008-1-050008-14
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8th International Conference on Global Resource Conservation (ICGRC) (19 Jul 2017 - 20 Jul 2017 : Malang, Indonesia)
Abstract
This study aimed to characterize the probiotic properties of lactic acid bacteria from the naturally fermented milk of Indonesia, namely dangke and dadih. Fifty-one representative lactic acid bacteria belonging to the species Lactobacillus Plantarum, Lactococcus lactis subsp. lactis and Enterococcus faecium were evaluated in vitro for potential probiotic properties based on their bile salt resistance, low pH tolerance, antimicrobial activity, antibiotic susceptibility and adherence to Caco-2 colon cancer cells. In addition, bacteriocin related gene (plantaricin A), bile salt hydrolase (bsh) and mannose-specific adhesin (msa) genes in the genome of lactobacilli were also examined. None of the dangke isolates, which belonged to the species L. lactis subsp. lactis tolerated low pH. However, eight of the isolates were able to grow in the presence of bile salts. It was observed that L. plantarum strain S1.30 and SL2.7 from dadih tolerated low pH, survived bile salt concentrations and were resistant to vancomycin. Furthermore, these strains also contained bacteriocin regulating gene (plantaricin A) and msa and bsh genes in their genome. However, only the strain S1.30 exhibited optimal antimicrobial activity against the selected pathogens and was able to adhere to Caco-2 cells by up to 82.24±0.14%. Antagonistic activity of L. lactis subsp. lactis from dadih and dangke was not detected. However, 73.94±1.26% adherence to Caco-2 cells was demonstrated by L. lactis subsp. lactis strain SL3.34 sourced from dangke. These results suggest that Lactobacillus plantarum strain S1.30 associated with dadih fulfilled the in vitro probiotic criteria and could be exploited for further in vivo evaluation. In addition, dadih was an effective probiotic carrier compared to dangke.
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Copyright 2017 The author(s)