A primer extension denaturing high-performance liquid chromatography method for the identification of three ABCC2 genetic polymorphisms

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dc.contributor.authorWestley, I.
dc.contributor.authorColler, J.
dc.contributor.authorWard, M.
dc.contributor.authorEvans, A.
dc.contributor.authorMorris, R.
dc.contributor.authorSallustio, B.
dc.date.issued2014
dc.description.abstractBackground: A number of single nucleotide polymorphisms have been described in the ABCC2 gene that alters drug disposition. The aim of the study was to develop a primer extension denaturing high-performance liquid chromatography (PE-dHPLC) assay to determine three common variants of the ABCC2 gene in the promoter region (−24C>T), exon 10 (1249G>A), and exon 28 (3972C>T). Methods: Polymerase chain reactions (PCRs) were used to isolate the area of interest in the ABCC2 gene. A comparison of the PCR product was performed between sequencing and dHPLC. Results: A 100% identity match was achieved between groups and allowed for a quick and accurate method to determine three single nucleotide polymorphisms in a single extension reaction. This assay is the first of its type to determine three ABCC2 variants by dHPLC
dc.description.statementofresponsibilityIan S. Westley, Janet K. Coller, Michael B. Ward, Allan M. Evans, Raymond G. Morris, and Benedetta C. Sallustio
dc.identifier.citationJournal of Liquid Chromatography and Related Technologies, 2014; 37(9):1249-1256
dc.identifier.doi10.1080/10826076.2013.789796
dc.identifier.issn1082-6076
dc.identifier.issn1520-572X
dc.identifier.orcidColler, J. [0000-0002-8273-5048]
dc.identifier.orcidSallustio, B. [0000-0002-0186-3073]
dc.identifier.urihttp://hdl.handle.net/2440/102144
dc.language.isoen
dc.publisherTaylor and Francis
dc.rightsCopyright © Taylor & Francis Group, LLC
dc.source.urihttps://doi.org/10.1080/10826076.2013.789796
dc.subjectABCC2; genotype; polymerase chain reaction; polymorphisms; PE-dHPLC; single nudeotide polymorphism
dc.titleA primer extension denaturing high-performance liquid chromatography method for the identification of three ABCC2 genetic polymorphisms
dc.typeJournal article
pubs.publication-statusPublished

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