A physical map of the chromosomal region determining O-antigen biosynthesis in Vibrio cholerae 01

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dc.contributor.authorWard, H.
dc.contributor.authorMorelli, G.
dc.contributor.authorKamke, M.
dc.contributor.authorMorona, R.
dc.contributor.authorYeadon, J.
dc.contributor.authorHackett, J.
dc.contributor.authorManning, P.
dc.date.issued1987
dc.description.abstractWe have previously described the cosmid cloning of the genes determining the biosynthesis of the Inaba and Ogawa O-antigens of the lipopolysaccharides of Vibrio cholerae 01 (Manning et al., 1986). By Southern hybridization analysis of chromosomal and cosmid DNA, and heteroduplex analysis between the clones we have been able to precisely define the region of contiguous chromosomal DNA in the vicinity of the O-antigen-encoding region. These data and comparison of end points of clones and of deletion derivatives demonstrate that at least 16kb of a 19-kb SstI fragment is required to encode O-antigen biosynthesis. Expression of O-antigen is independent of the orientation of this SstI fragment with respect to cloning vectors suggesting that its regulatory region has been cloned intact. No detectable differences were observed in the restriction patterns of the Inaba and Ogawa coding regions implying that only minor changes are involved when serotype conversion (Inaba to Ogawa or vice versa) occurs. Bhaskaran [Ind. J. Med. Res. 47 (1959) 253-260] originally defined this region associated with O-antigen biosynthesis oag; however, to be consistent with other organisms [Hitchcock et al., J. Bacteriol. 166 (1986) 699-705], it is suggested this be changed to rfb.
dc.description.statementofresponsibilityH.M. Ward, C. Morelli, M. Kamke, R. Morona, J. Yeadon, J.A. Hackett and P.A. Manning
dc.identifier.citationGene, 1987; 55(2-3):197-204
dc.identifier.doi10.1016/0378-1119(87)90280-0
dc.identifier.issn0378-1119
dc.identifier.issn1879-0038
dc.identifier.orcidWard, H. [0000-0002-3831-1205]
dc.identifier.orcidMorona, R. [0000-0001-7009-7440]
dc.identifier.urihttp://hdl.handle.net/2440/81805
dc.language.isoen
dc.publisherElsevier
dc.rights© 1987.
dc.source.urihttps://doi.org/10.1016/0378-1119(87)90280-0
dc.subjectcosmid
dc.subjectelectron microscopy
dc.subjectheteroduplex analysis
dc.subjectLipopolysaccharide
dc.subjectrecombinant DNA
dc.subjectserotype
dc.titleA physical map of the chromosomal region determining O-antigen biosynthesis in Vibrio cholerae 01
dc.typeJournal article
pubs.publication-statusPublished

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