99mTc-alafosfalin: an antibiotic peptide infection imaging agent

Simple item page
dc.contributor.authorTsopelas, C.
dc.contributor.authorPenglis, S.
dc.contributor.authorRuszkiewicz, A.
dc.contributor.authorBartholomeusz, F.
dc.date.issued2003
dc.description.abstractThe radiolabeled antibiotic peptide (99m)Tc-alafosfalin was assessed as an infection imaging agent in a rat model by comparison with (99m)Tc-DTPA and (99m)Tc-leukocytes. (99m)Tc-alafosfalin was prepared via an instant cold kit and (99m)Tc-leukocytes were prepared using (99m)Tc-stannous fluoride colloid in an ex vivo labeling procedure of whole blood. In separate experiments, the three radiotracers were administered to rats infected with staphylococcus aureus. Quantitative biodistribution studies were performed as well as scintigraphic images and histopathology. (99m)Tc-alafosfalin is a stable product, obtained in high radiochemical purity (>95%). This agent was mainly renally excreted, with low liver, spleen and bone uptake, and resulted in a mean ratio of infected/non-infected thighs of 4.3/1.0 at 4 hr post radiotracer injection. (99m)Tc-DTPA gave a corresponding ratio of 1.9/1.0 and (99m)Tc-leukocytes gave 20.0/1.0 at the same time point. An in vitro assay found the level of (99m)Tc-alafosfalin binding to staphylococcus aureas higher than (99m)Tc-DTPA (10% versus 1% respectively). (99m)Tc-alafosfalin accumulates at sites of infection in a rat model better than the perfusion molecule (99m)Tc-DTPA, yet less than (99m)Tc-leukocytes. The distribution characteristics of this (99m)Tc-antibiotic peptide would be an advantage in imaging abdominal and soft tissue infection.
dc.description.statementofresponsibilityC Tsopelas, S Penglis, A Ruszkiewicz, F.D.L Bartholomeusz
dc.identifier.citationNuclear Medicine and Biology, 2003; 30(2):169-175
dc.identifier.doi10.1016/S0969-8051(02)00348-7
dc.identifier.issn0969-8051
dc.identifier.issn1872-9614
dc.identifier.orcidRuszkiewicz, A. [0000-0001-9052-4948]
dc.identifier.urihttp://hdl.handle.net/2440/5793
dc.language.isoen
dc.publisherElsevier Science Inc
dc.source.urihttps://doi.org/10.1016/s0969-8051(02)00348-7
dc.subjectLeukocytes
dc.subjectAnimals
dc.subjectRats
dc.subjectRats, Sprague-Dawley
dc.subjectBacterial Infections
dc.subjectStaphylococcal Infections
dc.subjectMyositis
dc.subjectTechnetium
dc.subjectTechnetium Tc 99m Pentetate
dc.subjectOrganotechnetium Compounds
dc.subjectAlanine
dc.subjectPeptides
dc.subjectAnti-Bacterial Agents
dc.subjectRadiopharmaceuticals
dc.subjectRadionuclide Imaging
dc.subjectWhole-Body Counting
dc.subjectOrgan Specificity
dc.subjectTissue Distribution
dc.subjectFemale
dc.title99mTc-alafosfalin: an antibiotic peptide infection imaging agent
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Pathology publications