Imaging blood vessels in the zebrafish
Date
2010
Authors
Kamei, M.
Isogai, S.
Pan, W.
Weinstein, B.M.
Editors
Detrich, H.W.
Westerfield, M.
Zon, L.I.
Westerfield, M.
Zon, L.I.
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Book chapter
Citation
The Zebrafish: Cellular and Developmental Biology, Part A, 2010 / Detrich, H.W., Westerfield, M., Zon, L.I. (ed./s), vol.100, Ch.2, pp.27-54
Statement of Responsibility
Makoto Kamei, Sumio Isogai, Weijun Pan, and Brant M. Weinstein
Conference Name
Abstract
Understanding on the mechanisms of vascular branching morphogenesis has become a subject of enormous scientific and clinical interest. Zebrafish, which have small, accessible, transparent embryos and larvae, provides a unique living animal model to facilitating high-resolution imaging on ubiquitous and deep localization of vessels within embryo development and also in adult tissues. In this chapter, we have summarized various methods for vessel imaging in zebrafish, including in situ hybridization for vascular-specific genes, resin injection- or dye injection-based vessel visualization, and alkaline phosphatase staining. We also described detail protocols for live imaging of vessels by microangiography or using various transgenic zebrafish lines.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
Copyright © 2010 Elsevier Inc. All rights reserved.