Binding of chromium(VI) to histones: Implications for chromium(VI)-induced genotoxicity

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dc.contributor.authorLevina, A.
dc.contributor.authorHarris, H.
dc.contributor.authorLay, P.
dc.date.issued2006
dc.descriptionThe original publication can be found at www.springerlink.com
dc.description.abstractThe first evidence has been obtained for Cr(VI) (chromate) binding to isolated calf thymus (CT) histones under physiological conditions (pH 7.4, Cl− concentration 152 mM, 310 K). No significant Cr(VI) binding under the same conditions was observed for other extracellular and intracellular proteins, including albumin, apo-transferrin and G-actin, as well as for CT DNA. The mode of Cr(VI) binding to histones was studied by vibrational, electronic and X-ray absorption (X-ray absorption near-edge structure and X-ray absorption fine structure) spectroscopies and molecular mechanics calculations. A proposed binding mechanism includes electrostatic interactions of CrO4 2− with protonated Lys and Arg residues of histones, as well as the formation of hydrogen bonds with the protein backbone. Similarly, Cr(VI) can bind to nuclear localization signals (typically, Lys- and Arg-rich fragments) of other nuclear proteins. Selective binding of Cr(VI) to newly synthesized nuclear proteins (including histones) in the cytoplasm is likely to be responsible for the active transport of Cr(VI) into the nuclei of living cells.
dc.description.statementofresponsibilityAviva Levina, Hugh H. Harris and Peter A. Lay
dc.identifier.citationJournal of Biological Inorganic Chemistry, 2006; 11(2):225-234
dc.identifier.doi10.1007/s00775-005-0068-3
dc.identifier.issn0949-8257
dc.identifier.issn1432-1327
dc.identifier.orcidHarris, H. [0000-0002-3472-8628]
dc.identifier.urihttp://hdl.handle.net/2440/47034
dc.language.isoen
dc.publisherSpringer-Verlag
dc.relation.grantARC
dc.source.urihttp://www.springerlink.com/content/p4kl145nq741n65x/
dc.subjectChromium
dc.subjectCancer
dc.subjectHistones
dc.subjectNuclear localization signals
dc.subjectX-ray absorption spectroscopy
dc.titleBinding of chromium(VI) to histones: Implications for chromium(VI)-induced genotoxicity
dc.typeJournal article
pubs.publication-statusPublished

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