Imaging nuclear, endoplasmic reticulum and plasma membrane events in real time

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dc.contributor.authorBader, C.
dc.contributor.authorSorvina, A.
dc.contributor.authorSimpson, P.
dc.contributor.authorWright, P.
dc.contributor.authorStagni, S.
dc.contributor.authorPlush, S.
dc.contributor.authorMassi, M.
dc.contributor.authorBrooks, D.
dc.date.issued2016
dc.description.abstractLive cell imaging can provide important information on cellular dynamics; however, the full utilisation of this technology has been hampered by the limitations of imaging reagents. Metal-based complexes have the potential to overcome many of the issues common to many current imaging agents. The rhenium (I)-based complex fac-[Re(CO)3 (1,10-phenanthroline)(4-pyridyltetrazolate)], herein referred to as ReZolve-ER(™) , shows promise as a live cell imaging agent with rapid cell uptake, low cytotoxicity, resistance to photobleaching and compatibility with multicolour imaging. ReZolve-ER(™) localised to the nuclear membrane/endoplasmic reticulum (ER) and allowed the detection of exocytotic events at the plasma membrane. Thus, we present a new imaging agent for monitoring live cell events in real time, which is ideal for imaging either short- or long-time courses.
dc.description.statementofresponsibilityChristie A. Bader, Alexandra Sorvina, Peter V. Simpson, Phillip J. Wright, Stefano Stagni, Sally E. Plush, Massimiliano Massi and Douglas A. Brooks
dc.identifier.citationFEBS Letters, 2016; 590(18):3051-3060
dc.identifier.doi10.1002/1873-3468.12365
dc.identifier.issn0014-5793
dc.identifier.issn1873-3468
dc.identifier.orcidBrooks, D. [0000-0001-9098-3626]
dc.identifier.urihttp://hdl.handle.net/2440/105614
dc.language.isoen
dc.publisherWiley-Blacwell
dc.relation.granthttp://purl.org/au-research/grants/arc/FT130100033
dc.rights© 2016 Federation of European Biochemical Societies
dc.source.urihttps://doi.org/10.1002/1873-3468.12365
dc.subjectEndoplasmic reticulum; live cell imaging; rhenium
dc.titleImaging nuclear, endoplasmic reticulum and plasma membrane events in real time
dc.typeJournal article
pubs.publication-statusPublished

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