Cytokine receptor expression in human lymphoid tissue: analysis by fluorescence microscopy

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dc.contributor.authorZola, H.
dc.contributor.authorRidings, J.
dc.contributor.authorWeedon, H.
dc.contributor.authorFusco, M.
dc.contributor.authorByard, R.
dc.contributor.authorMacardle, P.
dc.date.issued1994
dc.description.abstractA highly-sensitive flourescence method, capable of detecting cytokine receptors present at low concentrations (around I DO molecules per cell) by flow cytometry, was adapted for use on tissue sections. This method was used to examine the expression of several cytokine receptors in lymphoid ti ss ues. lL-2 receptors were distributed broadly, with higher concentrations in T cell areas. lL-1 receptor Type I was detected in T cell areas and in the follicular mantle, and was strongly expressed on vasc ular endothelium. IL-6 receptor was found at very low concentration, both within and outside germinal centres. The gp 130 molecule, which is involved in the functional receptor complex for IL-6 and several other cytokines, was present at higher concentrations, particularly in the germinal centre. Analysis of receptor expression in secondary lymphoid tissue provides evidence bearing on the physiological roles of cytokines, as these tissues contain cells at various stages of physiological activation located in well-defined functional zones.
dc.description.statementofresponsibilityHeddy Zola, Jodie Ridings, Helen Weedon, Michael Fusco, Roger W. Byard, Peter J. Macardle
dc.identifier.citationDisease Markers, 1994; 12(2):147-165
dc.identifier.doi10.1155/1994/563543
dc.identifier.issn0278-0240
dc.identifier.issn1875-8630
dc.identifier.orcidByard, R. [0000-0002-0524-5942]
dc.identifier.urihttp://hdl.handle.net/2440/7199
dc.language.isoen
dc.publisherHindawi Publishing Corporation
dc.rightsCopyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttps://doi.org/10.1155/1994/563543
dc.subjectCytokine receptors; Interleukin receptors; Tissue staining; Cy3 fluorescence
dc.titleCytokine receptor expression in human lymphoid tissue: analysis by fluorescence microscopy
dc.typeJournal article
pubs.publication-statusPublished

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