The level of proBDNF in blood lymphocytes is correlated with that in the brain of rats with photothrombotic ischemic stroke

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dc.contributor.authorLuo, H.Y.
dc.contributor.authorRahman, M.
dc.contributor.authorBobrovskaya, L.
dc.contributor.authorZhou, X.F.
dc.date.issued2019
dc.description.abstractStroke is accompanied by severe inflammation in the brain. The role of mature brain-derived neurotrophic factor (mBDNF) in ischemic stroke has received intensive attention, but the function of its precursor proBDNF is less understood. Recent studies showed that mBDNF and proBDNF in the ischemic brain are upregulated, but the significance of mBDNF and proBDNF in the lymphocytes in ischemic stroke is not known. Here, we propose that the expression levels of mBDNF and proBDNF in lymphocytes correlate with those in the brain after ischemic stroke and therefore can be surrogate markers for the ischemic brain. Using a photothrombotic model in rats and ELISA assay technique, we found that proBDNF and mBDNF in peripheral lymphocytes were upregulated but produced differential time courses after ischemia. The levels of mBDNF and proBDNF in lymphocytes at early stages of stroke (1 day), showed a strong positive correlation with those in the brain. The levels of p75, sortilin, were also increased in a time-dependent manner after ischemic stroke; however, the levels of p-TrkB in the ischemic brain at 6 h, 1 and 3 days were significantly reduced in the brain. The present study suggests that the levels of proBDNF and mBDNF in the blood lymphocytes in acute ischemic stroke reflect those in the brain at early stages.
dc.description.statementofresponsibilityHai-Yun Luo, Mehreen Rahman, Larisa Bobrovskaya, Xin-Fu Zhou
dc.identifier.citationNeurotoxicity Research, 2019; 36(1):49-57
dc.identifier.doi10.1007/s12640-019-00022-0
dc.identifier.issn1029-8428
dc.identifier.issn1476-3524
dc.identifier.orcidZhou, X.F. [0000-0002-8687-0175]
dc.identifier.urihttps://hdl.handle.net/2440/132148
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.grantNHMRC
dc.rights© Springer Science+Business Media, LLC, part of Springer Nature 2019
dc.source.urihttps://doi.org/10.1007/s12640-019-00022-0
dc.subjectproBDNF; mature BDNF; TrkB; sortilin; p75; photothrombotic stroke; lymphocytes
dc.subject.meshLymphocytes
dc.subject.meshAnimals
dc.subject.meshRats, Sprague-Dawley
dc.subject.meshBrain Ischemia
dc.subject.meshDisease Models, Animal
dc.subject.meshBrain-Derived Neurotrophic Factor
dc.subject.meshProtein Precursors
dc.subject.meshUp-Regulation
dc.subject.meshStroke
dc.subject.meshBiomarkers
dc.titleThe level of proBDNF in blood lymphocytes is correlated with that in the brain of rats with photothrombotic ischemic stroke
dc.typeJournal article
pubs.publication-statusPublished

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