Modulation of respiratory syncytial virus-induced prostaglandin E₂ production by n-3 long-chain polyunsaturated fatty acids in human respiratory epithelium

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dc.contributor.authorBryan, D.
dc.contributor.authorHart, P.
dc.contributor.authorForsyth, K.
dc.contributor.authorGibson, R.
dc.date.issued2005
dc.description.abstractInfection with respiratory syncytial virus (RSV) results in substantial infant morbidity and has been associated with the subsequent development of childhood asthma. Inflammatory mediators produced by both the epithelium and tissue leukocytes during RSV infection stimulate the release of chemotactic factors by the respiratory epithelium and the subsequent influx of inflammatory cells, predominantly neutrophils. We investigated the production of inflammatory mediators [prostaglandin E ₂ (PGE ₂), interleukin (IL)-1β, tumor necrosis factor α] and chemokines [IL-8, RANTES (regulation on activation, normal T cell expressed and secreted)] by alveolar epithelial cells in response to RSV infection. Infection of a human alveolar epithelial transformed cell line (A549 cells) with live RSV substantially increased production of PGE ₂, IL-8, and RANTES. By altering cell membrane FA through incorporation of the long-chain PUFA (LCPUFA) arachidonic acid, EPA, and DHA, we were subsequently able to significantly modulate PGE ₂ production by the infected epithelium. Because of the dynamic nature of the effects of PGE ₂ on lung function, regulation of this prostaglandin during RSV infection by n-3 LCPUFA has the potential to significantly alter the disease process.
dc.description.statementofresponsibilityDani-Louise Bryan, Prue Hart, Kevin Forsyth and Robert Gibson
dc.identifier.citationLipids, 2005; 40(10):1007-1011
dc.identifier.doi10.1007/s11745-005-1463-4
dc.identifier.issn0024-4201
dc.identifier.issn1558-9307
dc.identifier.orcidForsyth, K. [0000-0002-2748-0353]
dc.identifier.orcidGibson, R. [0000-0002-8750-525X]
dc.identifier.urihttp://hdl.handle.net/2440/17415
dc.language.isoen
dc.publisherAmer Oil Chemists Soc A O C S Press
dc.source.urihttps://doi.org/10.1007/s11745-005-1463-4
dc.subjectRespiratory Mucosa
dc.subjectCell Line, Tumor
dc.subjectCell Membrane
dc.subjectHumans
dc.subjectRespiratory Syncytial Virus, Human
dc.subjectFatty Acids, Omega-3
dc.subjectDocosahexaenoic Acids
dc.subjectEicosapentaenoic Acid
dc.subjectArachidonic Acid
dc.subjectDinoprostone
dc.subjectPhospholipids
dc.subjectInterleukin-8
dc.subjectChemokine CCL5
dc.titleModulation of respiratory syncytial virus-induced prostaglandin E₂ production by n-3 long-chain polyunsaturated fatty acids in human respiratory epithelium
dc.title.alternativeModulation of respiratory syncytial virus-induced prostaglandin E(2) production by n-3 long-chain polyunsaturated fatty acids in human respiratory epithelium
dc.typeJournal article
pubs.publication-statusPublished

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