Instability of CII is needed for efficient switching between lytic and lysogenic development in bacteriophage 186

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dc.contributor.authorMurchland, I.M.
dc.contributor.authorAhlgren-Berg, N.
dc.contributor.authorPietsch, J.M.J.
dc.contributor.authorIsabel, A.
dc.contributor.authorDodd, I.B.
dc.contributor.authorShearwin, K.E.
dc.date.issued2020
dc.description.abstractThe CII protein of temperate coliphage 186, like the unrelated CII protein of phage λ, is a transcriptional activator that primes expression of the CI immunity repressor and is critical for efficient establishment of lysogeny. 186-CII is also highly unstable, and we show that in vivo degradation is mediated by both FtsH and RseP. We investigated the role of CII instability by constructing a 186 phage encoding a protease resistant CII. The stabilised-CII phage was defective in the lysis-lysogeny decision: choosing lysogeny with close to 100% frequency after infection, and forming prophages that were defective in entering lytic development after UV treatment. While lysogenic CI concentration was unaffected by CII stabilisation, lysogenic transcription and CI expression was elevated after UV. A stochastic model of the 186 network after infection indicated that an unstable CII allowed a rapid increase in CI expression without a large overshoot of the lysogenic level, suggesting that instability enables a decisive commitment to lysogeny with a rapid attainment of sensitivity to prophage induction.
dc.description.statementofresponsibilityIain M Murchland, Alexandra Ahlgren-Berg, Julian M J Pietsch, Alejandra Isabel, Ian B Dodd, Keith E Shearwin
dc.identifier.citationNucleic Acids Research, 2020; 48(21):1-12
dc.identifier.doi10.1093/nar/gkaa1065
dc.identifier.issn0305-1048
dc.identifier.issn1362-4962
dc.identifier.orcidIsabel, A. [0000-0002-4707-0140]
dc.identifier.orcidDodd, I.B. [0000-0003-2969-6841]
dc.identifier.orcidShearwin, K.E. [0000-0002-7736-2742]
dc.identifier.urihttps://hdl.handle.net/2440/133154
dc.language.isoen
dc.publisherOxford University Press
dc.relation.granthttp://purl.org/au-research/grants/arc/DP150103009
dc.relation.granthttp://purl.org/au-research/grants/arc/DP160101450
dc.rights© The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttps://doi.org/10.1093/nar/gkaa1065
dc.subjectEscherichia coli
dc.subjectColiphages
dc.subjectProphages
dc.subjectATP-Dependent Proteases
dc.subjectEndopeptidases
dc.subjectEscherichia coli Proteins
dc.subjectMembrane Proteins
dc.subjectViral Proteins
dc.subjectModels, Statistical
dc.subjectStochastic Processes
dc.subjectUltraviolet Rays
dc.subjectLysogeny
dc.subjectTranscriptional Activation
dc.subjectProtein Stability
dc.subjectProteolysis
dc.subject.meshEscherichia coli
dc.subject.meshColiphages
dc.subject.meshProphages
dc.subject.meshATP-Dependent Proteases
dc.subject.meshEndopeptidases
dc.subject.meshEscherichia coli Proteins
dc.subject.meshMembrane Proteins
dc.subject.meshViral Proteins
dc.subject.meshModels, Statistical
dc.subject.meshStochastic Processes
dc.subject.meshUltraviolet Rays
dc.subject.meshLysogeny
dc.subject.meshTranscriptional Activation
dc.subject.meshProtein Stability
dc.subject.meshProteolysis
dc.titleInstability of CII is needed for efficient switching between lytic and lysogenic development in bacteriophage 186
dc.typeJournal article
pubs.publication-statusPublished

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