Investigation of a gel-based delivery method for the administration of a live, attenuated Salmonella Typhimurium vaccine.

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dc.contributor.authorJia, S.
dc.contributor.authorMcWhorter, A.R.
dc.contributor.authorKhan, S.
dc.contributor.authorAndrews, D.M.
dc.contributor.authorUnderwood, G.J.
dc.contributor.authorChousalkar, K.K.
dc.date.issued2023
dc.description.abstractPoultry vaccines are often administered using water as a suspension media and applied using an oral or coarse spray method. Gel-based vaccine diluents have been developed as an alternative vaccine delivery method. Gels are more viscous, and droplets adhere more effectively to feathers giving the vaccine a longer time to be ingested (through preening). Application of gel diluents with live bacterial vaccines, however, is limited. The present study tested a gel diluent prepared in various media, using a live, attenuated Salmonella Typhimurium vaccine, Vaxsafe ST. Reconstitution in gel diluent did not negatively affect vaccine viability or motility. The invasive capacity of vaccine suspended in gel diluent into cultured intestinal epithelial cells was also tested. Results demonstrated that vaccine suspended in gel diluent retained invasiveness. Day old chicks were orally administered with Vaxsafe ST suspended in gel diluent to characterize in vivo colonization capacity of the vaccine. The results revealed that the VaxSafe ST suspended in gel diluent could efficiently colonize the caeca of chicks, which is needed for the development of effective immunity.
dc.description.statementofresponsibilitySiyuan Jia, Andrea R. McWhorter, Samiullah Khan, Daniel M. Andrews, Gregory J. Underwood, Kapil K. Chousalkar
dc.identifier.citationVeterinary Microbiology, 2023; 280:109721-109721
dc.identifier.doi10.1016/j.vetmic.2023.109721
dc.identifier.issn0378-1135
dc.identifier.issn1873-2542
dc.identifier.orcidJia, S. [0000-0002-8483-738X]
dc.identifier.orcidMcWhorter, A.R. [0000-0002-5793-1629]
dc.identifier.orcidKhan, S. [0000-0002-0292-2979]
dc.identifier.orcidChousalkar, K.K. [0000-0002-4134-3996]
dc.identifier.urihttps://hdl.handle.net/2440/137852
dc.language.isoen
dc.publisherElsevier BV
dc.relation.granthttp://purl.org/au-research/grants/arc/LP190100864
dc.rights© 2023 Elsevier B.V. All rights reserved.
dc.source.urihttps://doi.org/10.1016/j.vetmic.2023.109721
dc.subjectDelivery method
dc.subjectGel diluent
dc.subjectLive bacterial vaccine
dc.subjectPoultry
dc.subjectPrimary vaccination
dc.titleInvestigation of a gel-based delivery method for the administration of a live, attenuated Salmonella Typhimurium vaccine.
dc.typeJournal article
pubs.publication-statusPublished

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