β-Glucoside metabolism in Oenococcus oeni: Cloning and characterisation of the phospho-β-glucosidase bglD

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dc.contributor.authorCapaldo, A.
dc.contributor.authorWalker, M.
dc.contributor.authorFord, C.
dc.contributor.authorJiranek, V.
dc.date.issued2011
dc.description.abstractThe structural gene for a phospho-β-glucosidase from the oenologically important lactic acid bacterium (LAB) Oenococcus oeni has been cloned and its protein product characterised. This gene is found in a putative β-glucosidase operon of 2178 base pairs encoding 4 genes designated bglA to bglD. The bglA, B and C genes were not cloned and characterised, however, are thought to be phosphoenolpyruvate dependent phospho transferase system (PEP-PTS) components IIC, IIA and IIB which regulate the uptake, phosphorylation and translocation of β-glucosides across the cytoplasmic membrane. The cloned bglD was sequenced and expressed in Escherichia coli followed by purification. The purified bglD protein has 480 residues, a molecular mass of 55.5kDa and shows high homology to known phospho-β-glucosidases. bglD exhibited high activity towards the phosphorylated β-glucoside para-nitrophenol-β-d-glucopyranoside-6-phosphate with a pH optimum of 5.5 and maintained similar levels of activity between temperatures of 4°C and 40°C. The enzyme was not active against non-phosphorylated β-glucosides.
dc.description.statementofresponsibilityA. Capaldo, M.E. Walker, C.M. Ford and V. Jiranek
dc.identifier.citationFood Chemistry, 2011; 125(2):476-482
dc.identifier.doi10.1016/j.foodchem.2010.09.036
dc.identifier.issn0308-8146
dc.identifier.issn1873-7072
dc.identifier.orcidWalker, M. [0000-0002-6934-3787]
dc.identifier.orcidFord, C. [0000-0003-1617-2977]
dc.identifier.orcidJiranek, V. [0000-0002-9775-8963]
dc.identifier.urihttp://hdl.handle.net/2440/69986
dc.language.isoen
dc.publisherElsevier Sci Ltd
dc.rightsCopyright 2010 Elsevier Ltd. All rights reserved.
dc.source.urihttps://doi.org/10.1016/j.foodchem.2010.09.036
dc.subjectPhospho-b-glucosidase
dc.subjectOenococcus oeni
dc.subjectPhosphoenolpyruvate-phospho transferase
dc.subjectsystem
dc.titleβ-Glucoside metabolism in Oenococcus oeni: Cloning and characterisation of the phospho-β-glucosidase bglD
dc.title.alternativebeta-Glucoside metabolism in Oenococcus oeni: Cloning and characterisation of the phospho-beta-glucosidase bglD
dc.typeJournal article
pubs.publication-statusPublished

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