A Drosophila model to image phagosome maturation

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dc.contributor.authorShandala, T.
dc.contributor.authorLim, C.
dc.contributor.authorSorvina, A.
dc.contributor.authorBrooks, D.
dc.date.issued2013
dc.description.abstractPhagocytosis involves the internalization of extracellular material by invagination of the plasma membrane to form intracellular vesicles called phagosomes, which have functions that include pathogen degradation. The degradative properties of phagosomes are thought to be conferred by sequential fusion with endosomes and lysosomes; however, this maturation process has not been studied in vivo. We employed Drosophila hemocytes, which are similar to mammalian professional macrophages, to establish a model of phagosome maturation. Adult Drosophila females, carrying transgenic Rab7-GFP endosome and Lamp1-GFP lysosome markers, were injected with E. coli DH5α and the hemocytes were collected at 15, 30, 45 and 60 minutes after infection. In wild-type females, E. coli were detected within enlarged Rab7-GFP positive phagosomes at 15 to 45 minutes after infection; and were also observed in enlarged Lamp1-GFP positive phagolysosomes at 45 minutes. Two-photon imaging of hemocytes in vivo confirmed this vesicle morphology, including enlargement of Rab7-GFP and Lamp1-GFP structures that often appeared to protrude from hemocytes. The interaction of endosomes and lysosomes with E. coli phagosomes observed in Drosophila hemocytes was consistent with that previously described for phagosome maturation in human ex vivo macrophages. We also tested our model as a tool for genetic analysis using 14-3-3e mutants, and demonstrated altered phagosome maturation with delayed E. coli internalization, trafficking and/or degradation. These findings demonstrate that Drosophila hemocytes provide an appropriate, genetically amenable, model for analyzing phagosome maturation ex vivo and in vivo.
dc.description.statementofresponsibilityTetyana Shandala, Chiaoxin Lim, Alexandra Sorvina and Douglas A. Brooks
dc.identifier.citationCells, 2013; 2(2):188-201
dc.identifier.doi10.3390/cells2020188
dc.identifier.issn2073-4409
dc.identifier.issn2073-4409
dc.identifier.orcidBrooks, D. [0000-0001-9098-3626]
dc.identifier.urihttp://hdl.handle.net/2440/90256
dc.language.isoen
dc.publisherMDPI
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/631915
dc.rights© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
dc.source.urihttps://doi.org/10.3390/cells2020188
dc.subjectDrosophila; ex vivo; in vivo; hemocytes; phagocytosis; E. coli; Rab7 GTPase; Lamp1; 14-3-3 protein
dc.titleA Drosophila model to image phagosome maturation
dc.typeJournal article
pubs.publication-statusPublished

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