PPAR-alpha cloning, expression, and characterization
Date
2013
Authors
Ngo, S.N.
McKinnon, R.A.
Editors
Badr, M.Z.
Youssef, J.A.
Youssef, J.A.
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Book chapter
Citation
Peroxisome Proliferator-Activated Receptors (PPARs): methods and protocols, 2013 / Badr, M.Z., Youssef, J.A. (ed./s), vol.952, Ch.2, pp.7-34
Statement of Responsibility
Suong N. T. Ngo and Ross A. McKinnon
Conference Name
Abstract
Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems.
School/Discipline
Dissertation Note
Provenance
Description
Access Status
Rights
© Springer Science+Business Media New York 2013