Evaluation of an image analysis device (APAS) for screening urine cultures

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dc.contributor.authorGlasson, J.
dc.contributor.authorHill, R.
dc.contributor.authorSummerford, M.
dc.contributor.authorGiglio, S.
dc.contributor.editorOnderdonk, A.
dc.date.issued2016
dc.description.abstractWhile advancements have been made in some areas of pathology with diagnostic materials being screened using image analysis technologies, the reporting of cultures from agar plates remains a manual process. We compared the results for 2,163 urine cultures read by a reference panel of microbiologists, by the routine laboratory process, and by an automated plate reading system, APAS (LBT Innovations Ltd., South Australia). APAS detected colonies with a sensitivity of 99.1% and a specificity of 99.3% on blood agar, while on MacConkey agar, the colony detection sensitivity was 99.4% with a specificity of 99.3%. The device's ability to enumerate growth had an accuracy of 89.2%, and the morphological identification of colonies showed a high level of performance for the colony types typical of Escherichia coli and other enteric bacilli. On blood agar, lactose-fermenting colonies were morphologically identified with a sensitivity of 98.9%, while on MacConkey agar they were identified with a sensitivity of 99.2%. In this first clinical evaluation, APAS demonstrated high performance in the detection, enumeration, and colony classification of isolates compared with that for conventional plate-reading methods. The device found all cases reported by the laboratory and detected the most commonly encountered organisms found in urinary tract infections.
dc.description.statementofresponsibilityJohn Glasson, Rhys Hill, Michael Summerford, Steven Giglio
dc.identifier.citationJournal of Clinical Microbiology, 2016; 54(2):300-304
dc.identifier.doi10.1128/JCM.02365-15
dc.identifier.issn0095-1137
dc.identifier.issn1098-660X
dc.identifier.urihttp://hdl.handle.net/2440/113627
dc.language.isoen
dc.publisherAmerican Society for Microbiology
dc.rightsCopyright © 2016 Glasson et al. This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.source.urihttps://doi.org/10.1128/jcm.02365-15
dc.subjectBacteriological techniques
dc.titleEvaluation of an image analysis device (APAS) for screening urine cultures
dc.typeJournal article
pubs.publication-statusPublished

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