Characterization and expression of the cytochrome P450 gene family in diamondback moth, Plutella xylostella (L.)

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dc.contributor.authorYu, L.
dc.contributor.authorTang, W.
dc.contributor.authorHe, W.
dc.contributor.authorMa, X.
dc.contributor.authorVasseur, L.
dc.contributor.authorBaxter, S.
dc.contributor.authorYang, G.
dc.contributor.authorHuang, S.
dc.contributor.authorSong, F.
dc.contributor.authorYou, M.
dc.date.issued2015
dc.description.abstractCytochrome P450 monooxygenases are present in almost all organisms and can play vital roles in hormone regulation, metabolism of xenobiotics and in biosynthesis or inactivation of endogenous compounds. In the present study, a genome-wide approach was used to identify and analyze the P450 gene family of diamondback moth, Plutella xylostella, a destructive worldwide pest of cruciferous crops. We identified 85 putative cytochrome P450 genes from the P. xylostella genome, including 84 functional genes and 1 pseudogene. These genes were classified into 26 families and 52 subfamilies. A phylogenetic tree constructed with three additional insect species shows extensive gene expansions of P. xylostella P450 genes from clans 3 and 4. Gene expression of cytochrome P450s was quantified across multiple developmental stages (egg, larva, pupa and adult) and tissues (head and midgut) using P. xylostella strains susceptible or resistant to insecticides chlorpyrifos and fiprinol. Expression of the lepidopteran specific CYP367s predominantly occurred in head tissue suggesting a role in either olfaction or detoxification. CYP340s with abundant transposable elements and relatively high expression in the midgut probably contribute to the detoxification of insecticides or plant toxins in P. xylostella. This study will facilitate future functional studies of the P. xylostella P450s in detoxification.
dc.description.statementofresponsibilityLiying Yu, Weiqi Tang, Weiyi He, Xiaoli Ma, Liette Vasseur, Simon W. Baxter, Guang Yang, Shiguo Huang, Fengqin Song, Minsheng You
dc.identifier.citationScientific Reports, 2015; 5(1):8952-1-8952-9
dc.identifier.doi10.1038/srep08952
dc.identifier.issn2045-2322
dc.identifier.issn2045-2322
dc.identifier.orcidBaxter, S. [0000-0001-5773-6578]
dc.identifier.urihttp://hdl.handle.net/2440/94181
dc.language.isoen
dc.publisherNature
dc.relation.grantARC
dc.rightsThis work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
dc.source.urihttps://doi.org/10.1038/srep08952
dc.subjectAnimals
dc.subjectLepidoptera
dc.subjectCytochrome P-450 Enzyme System
dc.subjectInsecticides
dc.subjectPhylogeny
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectLarva
dc.subjectPupa
dc.subjectInsecticide Resistance
dc.subjectMultigene Family
dc.subjectInactivation, Metabolic
dc.titleCharacterization and expression of the cytochrome P450 gene family in diamondback moth, Plutella xylostella (L.)
dc.typeJournal article
pubs.publication-statusPublished

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