Endothelial denudation of isolated human internal mammary artery segments

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dc.contributor.authorLamin, V.
dc.contributor.authorWorthington, M.
dc.contributor.authorEdwards, J.
dc.contributor.authorViana, F.
dc.contributor.authorStuklis, R.
dc.contributor.authorWilson, D.
dc.contributor.authorBeltrame, J.
dc.date.issued2016
dc.description.abstractBackground: Endothelial denudation is an important approach to evaluate the role of the endothelium in vascular reactivity studies. Although approaches to remove the endothelium are well established in animal models, these methods have proved difficult to effectively translate to remnants of human Internal Mammary Artery (IMA) obtained during coronary bypass. This study sought to identify the optimal technique for endothelial denudation of IMA while preserving vascular contractile responses. Methods: IMA segments were subject to endothelial denudation using one of the following techniques: (1) surface abrasion, rubbing with a stainless steel wire, (2) vasoconstriction abrasion or (3) shear abrasion via infusion of an effervescent solution. Following intervention, IMA segments were evaluated by: (1) histochemistry to quantify structural damage and endothelial cell abundance and (2) functional endothelium-dependent vasodilator response using vascular myography in an organ bath preparation. Results: Vasoconstriction abrasion removed endothelial cells and caused disruption of the internal elastic lamina, these vessels failed to respond to the vasoconstrictor Phenylephrine (PE) or the endothelium-dependent vasodilator A23187. Surface abrasion alone was incomplete in removing endothelial cells, vessel vasodilated partially when challenged with A23187 in the presence of PE. Shear abrasion removed endothelial cells most effectively, as these pre-constricted vessels did not relax to A23187 but demonstrated increased sensitivity to PE. Conclusions: In this controlled comparative study assessing both structural and functional endpoints of endothelial denudation techniques, we have demonstrated that shear abrasion by infusion of an effervescent solution is the optimal technique to remove the endothelium and preserve vascular function in human IMA.
dc.description.statementofresponsibilityVictor Lamin, Michael Worthington, James Edwards, Fabiano Viana, Robert Stuklis, David Wilson and John Beltrame
dc.identifier.citationCardiovascular Pharmacology: Open Access, 2016; 5(3):1000180-1-1000180-5
dc.identifier.doi10.4172/2329-6607.1000180
dc.identifier.issn2329-6607
dc.identifier.orcidBeltrame, J. [0000-0002-4294-6510]
dc.identifier.urihttp://hdl.handle.net/2440/123660
dc.language.isoen
dc.publisherLongdom Publishing
dc.rightsCopyright: © 2016 Lamin V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.source.urihttps://www.longdom.org/abstract/endothelial-denudation-of-isolated-human-internal-mammary-arterysegments-14041.html
dc.subjectInternal mammary artery; Endothelium denudation; Vascular reactivity
dc.titleEndothelial denudation of isolated human internal mammary artery segments
dc.typeJournal article
pubs.publication-statusPublished

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