Oyster viperin retains direct antiviral activity and its transcription occurs via a signalling pathway involving a heat-stable haemolymph protein

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dc.contributor.authorGreen, T.
dc.contributor.authorSpeck, P.
dc.contributor.authorGeng, L.
dc.contributor.authorRaftos, D.
dc.contributor.authorBeard, M.
dc.contributor.authorHelbig, K.
dc.date.issued2015
dc.description.abstractLittle is known about the response of non-model invertebrates, such as oysters, to viral infection. The vertebrate innate immune system detects virus-derived nucleic acids to trigger the type I interferon (IFN)-pathway, leading to the transcription of hundreds of IFN-stimulated genes (ISGs) that exert antiviral functions. Invertebrates were thought to lack the IFN-pathway based on the absence of IFN or ISGs encoded in model-invertebrate genomes. However, the oyster genome encodes many ISGs, including the well-described antiviral protein, viperin. In this study, we characterise oyster-viperin and show it localises to caveolin-1 and inhibits Dengue virus replication in a heterologous model. In a second set of experiments, we provide evidence that the hemolymph from poly(I:C)-injected oysters contains a heat-stable, protease-susceptible factor that induces hemocyte transcription of viperin mRNA in conjunction with upregulation of IFN-regulatory factor. Collectively, these results support the concept that oysters have antiviral systems that are homologous to the vertebrate IFN-pathway.
dc.description.statementofresponsibilityTimothy J. Green, Peter Speck, Lu Geng, David Raftos, Michael R. Beard and Karla J. Helbig
dc.identifier.citationJournal of General Virology, 2015; 96(12):3587-3597
dc.identifier.doi10.1099/jgv.0.000300
dc.identifier.issn0022-1317
dc.identifier.issn1465-2099
dc.identifier.orcidBeard, M. [0000-0002-4106-1016]
dc.identifier.urihttp://hdl.handle.net/2440/99331
dc.language.isoen
dc.publisherMicrobiology Society
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1053206
dc.rights© 2015 The Authors
dc.source.urihttps://doi.org/10.1099/jgv.0.000300
dc.subjectHemolymph
dc.subjectAnimals
dc.subjectDengue Virus
dc.subjectLipids
dc.subjectProteins
dc.subjectAntiviral Agents
dc.subjectVirus Replication
dc.subjectSignal Transduction
dc.subjectGene Expression Regulation
dc.subjectAmino Acid Sequence
dc.subjectMolecular Sequence Data
dc.subjectOstreidae
dc.subjectCaveolin 1
dc.subjectHot Temperature
dc.titleOyster viperin retains direct antiviral activity and its transcription occurs via a signalling pathway involving a heat-stable haemolymph protein
dc.typeJournal article
pubs.publication-statusPublished

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