Monoclonal antibodies generated by DNA immunization recognize CD2 from a broad range of primates

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dc.contributor.authorBrady, J.
dc.contributor.authorMannering, S.
dc.contributor.authorKireta, S.
dc.contributor.authorCoates, P.
dc.contributor.authorProietto, A.
dc.contributor.authorCowan, P.
dc.contributor.authord'Apice, A.
dc.contributor.authorLew, A.
dc.date.issued2009
dc.description© 2009 Australasian Society for Immunology Inc. All rights reserved.
dc.description.abstractUsing heterologous prime-boost (DNA immunization followed by immunization with transfected cells), we have generated depleting mouse anti-baboon CD2 monoclonal antibodies (mAb). These anti-CD2 mAb recognized a diverse range of primate CD2 from New World monkeys and Old World monkeys to humans and have potent immunosuppressive activity for human allo-MLR responses and anti-tetanus-toxoid recall responses. There was no upregulation of activation markers or release of cytokines when the mAb were incubated with human peripheral blood mononuclear cells. Using chimeric NOD-SCID IL2rγnull mice, the mAb were shown to deplete human and cynomolgus monkey T cells in vivo. These anti-CD2 mAb may therefore be important immunological tools in allo- and xenotransplantation.
dc.description.statementofresponsibilityJamie L. Brady, Stuart I. Mannering, Svjetlana Kireta, Patrick T. Coates, Anna I. Proietto, Peter J. Cowan, Anthony J. F. D'Apice and Andrew M. Lew
dc.identifier.citationImmunology and Cell Biology, 2009; 87(5):413-418
dc.identifier.doi10.1038/icb.2009.4
dc.identifier.issn0818-9641
dc.identifier.issn1440-1711
dc.identifier.urihttp://hdl.handle.net/2440/57085
dc.language.isoen
dc.publisherBlackwell Publishing Asia
dc.source.urihttps://doi.org/10.1038/icb.2009.4
dc.subjectCD2
dc.subjectdepleting
dc.subjectDNA
dc.subjecthumanized mice
dc.subjectmonoclonal
dc.subjectprimate
dc.titleMonoclonal antibodies generated by DNA immunization recognize CD2 from a broad range of primates
dc.typeJournal article
pubs.publication-statusPublished

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