Uterine epithelial cells synthesize granulocyte-macrophage colony-stimulating factor and interleukin-6 in pregnant and nonpregnant mice

Simple item page
dc.contributor.authorRobertson, S.A.
dc.contributor.authorMayrhofer, G.
dc.contributor.authorSeamark, R.F.
dc.date.issued1992
dc.description.abstractCytokine secretion by endometrial cells from estrous and mated mice was measured using specific bioassays. The granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-6 (IL-6) contents of uterine intraluminal fluid were elevated greater than 20-fold and 250-fold respectively following mating, and both cytokines were synthesized in abundance in vitro by uterine cells harvested at estrus and on Day 1 of pregnancy. Synthesis was not impaired in genetically lymphocyte-deficient nude, SCID, or beige mice. To determine the cellular origin of the cytokines, a panning technique employing monoclonal antibodies against a range of leukocyte and other lineage markers was used to isolate uterine cell subsets in vitro. These experiments identified glandular and/or luminal epithelial cells as the major source of GM-CSF and IL-6 in estrous and pregnant uteri. Stromal fibroblasts also synthesized IL-6, as did macrophages in mated mice. Epithelial cells harvested from midgestation uteri secreted GM-CSF and IL-6 in quantities similar to those of cells from estrous and mated mice. Bioactivities of both cytokines derived from epithelial cells were neutralized by specific antibodies, and size-exclusion chromatography of conditioned media from uterine cells revealed peaks of GM-CSF and IL-6 bioactivity with M(r) 23,000 and 23,000-26,000, respectively. Bioassay of luminal fluids and culture supernatants were negative for the cytokines interleukin-1, interleukin-2, interleukin-3, and tumor necrosis factor-alpha. These studies identify murine uterine epithelium as a potent source of the cytokines GM-CSF and IL-6, which we postulate have potentially important functions in pregnancy through actions on target cells in both the uterus and the conceptus.
dc.description.statementofresponsibilitySarah A. Robertson, Graham Mayrhofer, and Robert F. Seamark
dc.identifier.citationBiology of Reproduction, 1992; 46(6):1069-1079
dc.identifier.doi10.1095/biolreprod46.6.1069
dc.identifier.issn0006-3363
dc.identifier.issn1529-7268
dc.identifier.orcidRobertson, S.A. [0000-0002-9967-0084]
dc.identifier.urihttp://hdl.handle.net/2440/85261
dc.language.isoen
dc.publisherSociety for the Study of Reproduction
dc.rightsCopyright status unknown
dc.source.urihttps://doi.org/10.1095/biolreprod46.6.1069
dc.subjectUterus
dc.subjectEndometrium
dc.subjectEpithelium
dc.subjectCells, Cultured
dc.subjectAnimals
dc.subjectMice, Inbred BALB C
dc.subjectMice, Inbred C57BL
dc.subjectMice, Inbred CBA
dc.subjectMice
dc.subjectMice, Nude
dc.subjectMice, SCID
dc.subjectGranulocyte-Macrophage Colony-Stimulating Factor
dc.subjectInterleukin-6
dc.subjectCulture Media
dc.subjectImmunohistochemistry
dc.subjectEstrus
dc.subjectPregnancy
dc.subjectPregnancy, Animal
dc.subjectMolecular Weight
dc.subjectFemale
dc.titleUterine epithelial cells synthesize granulocyte-macrophage colony-stimulating factor and interleukin-6 in pregnant and nonpregnant mice
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Obstetrics and Gynaecology publications