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Extracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling

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dc.contributor.authorZhao, K.
dc.contributor.authorBleackley, M.
dc.contributor.authorChisanga, D.
dc.contributor.authorGangoda, L.
dc.contributor.authorFonseka, P.
dc.contributor.authorLiem, M.
dc.contributor.authorKalra, H.
dc.contributor.authorAl Saffar, H.
dc.contributor.authorKeerthikumar, S.
dc.contributor.authorAng, C.-S.
dc.contributor.authorAdda, C.G.
dc.contributor.authorJiang, L.
dc.contributor.authorYap, K.
dc.contributor.authorPoon, I.K.
dc.contributor.authorLock, P.
dc.contributor.authorBulone, V.
dc.contributor.authorAnderson, M.A.
dc.contributor.authorMathivanan, S.
dc.date.issued2019
dc.description.abstractExtracellular vesicles (EVs) are membranous vesicles that are released by cells. In this study, the role of the Endosomal Sorting Complex Required for Transport (ESCRT) machinery in the biogenesis of yeast EVs was examined. Knockout of components of the ESCRT machinery altered the morphology and size of EVs as well as decreased the abundance of EVs. In contrast, strains with deletions in cell wall biosynthesis genes, produced more EVs than wildtype. Proteomic analysis highlighted the depletion of ESCRT components and enrichment of cell wall remodelling enzymes, glucan synthase subunit Fks1 and chitin synthase Chs3, in yeast EVs. Interestingly, EVs containing Fks1 and Chs3 rescued the yeast cells from antifungal molecules. However, EVs from fks1∆ or chs3∆ or the vps23∆chs3∆ double knockout strain were unable to rescue the yeast cells as compared to vps23∆ EVs. Overall, we have identified a potential role for yeast EVs in cell wall remodelling.
dc.description.statementofresponsibilityKening Zhao, Mark Bleackley, David Chisanga, Lahiru Gangoda, Pamali Fonseka, Michael Liem, Hina Kalra, Haidar Al Saffar, Shivakumar Keerthikumar, Ching-Seng Ang, Christopher G. Adda, Lanzhou Jiang, Kuok Yap, Ivan K. Poon, Peter Lock, Vincent Bulone, Marilyn Anderson, Suresh Mathivanan
dc.identifier.citationCommunications Biology, 2019; 2(1):1-13
dc.identifier.doi10.1038/s42003-019-0538-8
dc.identifier.issn2399-3642
dc.identifier.issn2399-3642
dc.identifier.orcidBulone, V. [0000-0002-9742-4701] [0000-0003-2809-4160]
dc.identifier.urihttp://hdl.handle.net/2440/121541
dc.language.isoen
dc.publisherSpringer Nature
dc.relation.granthttp://purl.org/au-research/grants/arc/DP160100309
dc.relation.granthttp://purl.org/au-research/grants/arc/DE150101777
dc.relation.granthttp://purl.org/au-research/grants/arc/DP170102312
dc.relation.granthttp://purl.org/au-research/grants/arc/FT180100333
dc.rights© The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
dc.source.urihttps://doi.org/10.1038/s42003-019-0538-8
dc.subjectCell Wall
dc.subjectSaccharomyces cerevisiae
dc.subjectAntifungal Agents
dc.subjectProteomics
dc.subjectCell Survival
dc.subjectMutation
dc.subjectStress, Physiological
dc.subjectEndosomal Sorting Complexes Required for Transport
dc.subjectExtracellular Vesicles
dc.subjectCaspofungin
dc.titleExtracellular vesicles secreted by Saccharomyces cerevisiae are involved in cell wall remodelling
dc.typeJournal article
pubs.publication-statusPublished

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