Measurement of labile Cu in soil using stable isotope dilution and isotope ratio analysis by ICP-MS

Date

2004

Authors

Nolan, A.
Ma, Y.
Lombi, E.
McLaughlin, M.

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Journal article

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Analytical and Bioanalytical Chemistry, 2004; 380(5-6):789-797

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Annette L. Nolan, Yibing Ma, Enzo Lombi and Mike J. McLaughlin

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Abstract

Isotope dilution is a useful technique to measure the labile metal pool, which is the amount of metal in soil in rapid equilibrium (<7 days) with the soil solution. This is normally performed by equilibrating soil with a metal isotope, and sampling the labile metal pool by using an extraction (E value), or by growing plants (L value). For Cu, this procedure is problematic for E values, and impossible for L values, due to the short half-life of the 64Cu radioisotope (12.4 h), which makes access and handling very difficult. We therefore developed a technique using enriched 65Cu stable isotope and measurement of 63Cu/65Cu ratios by quadrupole inductively coupled plasma mass spectrometry (ICP-MS) to measure labile pools of Cu in soils using E value techniques. Mass spectral interferences in detection of 63Cu/65Cu ratios in soil extracts were found to be minimal. Isotope ratios determined by quadrupole ICP-MS compared well to those determined by high-resolution (magnetic sector) ICP-MS. E values determined using the stable isotope technique compared well to those determined using the radioisotope for both uncontaminated and Cu-contaminated soils.

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Link to a related website: https://digital.library.adelaide.edu.au/dspace/bitstream/2440/41458/1/McLaughlin_442_final.pdf, Open Access via Unpaywall

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© 2004 Springer, Part of Springer Science+Business Media

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