The genetic diversity of lactic acid producing bacteria in the equine gastrointestinal tract

Simple item page
dc.contributor.authoral Jassim, R.
dc.contributor.authorScott, P.
dc.contributor.authorTrebbin, A.
dc.contributor.authorTrott, D.
dc.contributor.authorPollitt, C.
dc.date.issued2005
dc.description.abstractSeventy-two lactic acid producing bacterial isolates (excluding streptococci) were cultured from the gastrointestinal tract of six horses. Two of the horses were orally dosed with raftilose to induce lactic acidosis and laminitis while the remaining four were maintained on a roughage diet. Near complete 16S rDNA was amplified by PCR from the genomic DNA of each isolate. Following RFLP analysis with the restriction enzymes MboI, HhaI and HinfI, the PCR products from the 18 isolates that produced L- and/or D-lactate were subsequently cloned and sequenced. DNA sequence analysis indicated that the majority of the isolates were closely related to species within the genus Lactobacillus, including Lactobacillus salivarius, Lactobacillus mucosae and Lactobacillus delbrueckii. Four isolates were closely related to Mitsuokella jalaludinii. Lactic acid producing bacteria (LAB) from the equine gastrointestinal tract was dominated by representatives from the genus Lactobacillus, but also included D-lactate-producing bacteria closely related to M. jalaludinii. Identification and characterization of LAB from the equine gastrointestinal tract should contribute to our understanding and management of fermentative acidosis, ulceration of the stomach and laminitis.
dc.description.statementofresponsibilityRafat A.M. Al Jassim, Paul T. Scott, Andrea L. Trebbin, Darren Trott, Christopher C. Pollitt
dc.identifier.citationFEMS Microbiology Letters, 2005; 248(1):75-81
dc.identifier.doi10.1016/j.femsle.2005.05.023
dc.identifier.issn0378-1097
dc.identifier.issn1574-6968
dc.identifier.orcidTrott, D. [0000-0002-8297-5770]
dc.identifier.urihttp://hdl.handle.net/2440/65528
dc.language.isoen
dc.publisherElsevier Science BV
dc.rights© 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
dc.source.urihttps://doi.org/10.1016/j.femsle.2005.05.023
dc.subjectLactic acid producing bacteria
dc.subjectLactobacillus
dc.subjectL. salivarius
dc.subjectL. mucosae
dc.subjectL. delbrueckii
dc.subjectMitsuokella jalaludinii
dc.subjectAcidosis
dc.titleThe genetic diversity of lactic acid producing bacteria in the equine gastrointestinal tract
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Animal and Veterinary Sciences publications