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Porous silicon films micropatterned with bioelements as supports for mammalian cells

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dc.contributor.authorSweetman, M.J.
dc.contributor.authorRonci, M.
dc.contributor.authorGhaemi, S.R.
dc.contributor.authorCraig, J.E.
dc.contributor.authorVoelcker, N.
dc.date.issued2012
dc.description.abstractPorous silicon (pSi) surfaces have been chemically patterned via a UV initiated hydrosilylation reaction of an alkene through a photomask, introducing chemical functionality in the exposed surface areas. A secondary, UV initiated hydrosilylation reaction with a second alkene of different functionality is performed to backfill the silicon hydride terminated regions on the surface, thereby affording patterned porous films with dual, surface chemistry. UV initiated hydrosilylations were performed using the alkene undecylenic acid N-hydroxysuccinimide (NHS) ester, and the pSi surfaces were stabilized by a second hydrosilylation reaction with a polyethylene glycol (PEG) appended alkene. NHS ester and PEG functionalized surfaces were used for the selective immobilization of the cell adhesion mediator protein fibronectin (FN), in the NHS-functional regions. Matrix-assisted laser desorption/ionization mass spectrometry imaging on the protein functionalized pSi surface confirmed the patterned conjugation of the FN to the NHS functionalized regions. Mammalian cells cultured on these surfaces showed attachment that was confined to the patterned areas of FN on the pSi surface
dc.identifier.citationAdvanced Functional Materials, 2012; 22(6):1158-1166
dc.identifier.doi10.1002/adfm.201102000
dc.identifier.issn1616-301X
dc.identifier.issn1616-3028
dc.identifier.urihttps://hdl.handle.net/1959.8/156699
dc.language.isoen
dc.publisherWiley-VCH Verlag GmbH
dc.relation.fundingFlinders University
dc.relation.fundingAustralian Research Council
dc.rightsCopyright 2012 Wiley
dc.source.urihttps://doi.org/10.1002/adfm.201102000
dc.subjectporous silicon
dc.subjecthydrosilylation
dc.subjectpatterning
dc.subjectcell culture
dc.subjectbiomaterials
dc.titlePorous silicon films micropatterned with bioelements as supports for mammalian cells
dc.typeJournal article
pubs.publication-statusPublished
ror.mmsid9915909523001831

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