Close allies in membrane protein research: cell-free synthesis and nanotechnology

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dc.contributor.authorShadiac, N.
dc.contributor.authorNagarajan, Y.
dc.contributor.authorWaters, S.
dc.contributor.authorHrmova, M.
dc.date.issued2013
dc.description.abstractMembrane proteins control fundamental processes that are inherent to nearly all forms of life such as transport of molecules, catalysis, signaling, vesicle fusion, sensing of chemical and physical stimuli from the environment, and cell-cell interactions. Membrane proteins are harbored within a non-equilibrium fluid-like environment of biological membranes that separate cellular and non-cellular environments, as well as in compartmentalized cellular organelles. One of the classes of membrane proteins that will be specifically treated in this article are transport proteins of plant origin, that facilitate material and energy transfer at the membrane boundaries. These proteins import essential nutrients, export cellular metabolites, maintain ionic and osmotic equilibriums and mediate signal transduction. The aim of this article is to report on the progress of membrane protein functional and structural relationships, with a focus on producing stable and functional proteins suitable for structural and biophysical studies. We interlink membrane protein production primarily through wheat-germ cell-free protein synthesis (WG-CFPS) with the growing repertoire of membrane mimicking environments in the form of lipids, surfactants, amphipathic surfactant polymers, liposomes and nanodiscs that keep membrane proteins soluble. It is hoped that the advancements in these fields could increase the number of elucidated structures, in particular those of plant membrane proteins, and contribute to bridging of the gap between structures of soluble and membrane proteins, the latter being comparatively low.
dc.description.statementofresponsibilityNadim Shadiac, Yagnesh Nagarajan, Shane Waters & Maria Hrmova
dc.identifier.citationMolecular Membrane Biology, 2013; 30(3):229-245
dc.identifier.doi10.3109/09687688.2012.762125
dc.identifier.issn0968-7688
dc.identifier.issn1464-5203
dc.identifier.orcidHrmova, M. [0000-0002-3545-0605]
dc.identifier.urihttp://hdl.handle.net/2440/80198
dc.language.isoen
dc.publisherTaylor & Francis Ltd
dc.relation.grantARC
dc.relation.granthttp://purl.org/au-research/grants/arc/DP120100900
dc.relation.granthttp://purl.org/au-research/grants/arc/LP120100201
dc.relation.granthttp://purl.org/au-research/grants/arc/LP120100201
dc.rights© 2013 Informa UK, Ltd.
dc.source.urihttps://doi.org/10.3109/09687688.2012.762125
dc.subjectAmphipathic polymers
dc.subjectbiophysics
dc.subjectfunction and structure of membrane proteins
dc.subjectinteractomes
dc.subjectnanoparticles
dc.subjectphospholipid bilayers
dc.subjectmembrane protein reconstitution
dc.subjecttransporters
dc.subjectsurfactants
dc.titleClose allies in membrane protein research: cell-free synthesis and nanotechnology
dc.typeJournal article
pubs.publication-statusPublished

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