Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions
Date
2014
Authors
Mody, K.
Mahony, D.
Cavallaro, A.
Stahr, F.
Qiao, S.
Mahony, T.
Mitter, N.
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Journal article
Citation
International Journal of Pharmaceutics, 2014; 465(1-2):325-332
Statement of Responsibility
Karishma T. Mody, Donna Mahony, Antonino S. Cavallaro, Frances Stahr Shi Zhang Qiao, Timothy J. Mahony, Neena Mitter
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Abstract
Amino functionalised mesoporous silica nanoparticles (AM-41) have been identified as a promising vaccine delivery material. The capacity of AM-41 to stabilise vaccine components at ambient temperature (23-27°C) was determined by adsorbing the model antigen ovalbumin (OVA) to AM-41 particles (OVA-41). The OVA-41 was successfully freeze-dried using the excipients 5% trehalose and 1% PEG8000. The immunological activity of OVA and the nanoparticle structure were maintained following two months storage at ambient temperature. The results of immunisation studies in mice with reconstituted OVA-41 demonstrated the induction of humoral and cell-meditated immune responses. The capacity of AM-41 particles to facilitate ambient storage of vaccine components without the loss of immunological potency will underpin the further development of this promising vaccine delivery platform.
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© 2014 Elsevier B.V. All rights reserved.