Generation of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family

Simple item page
dc.contributor.authorTong, J.
dc.contributor.authorLee, K.M.
dc.contributor.authorLiu, X.
dc.contributor.authorNefzger, C.M.
dc.contributor.authorVijayakumar, P.
dc.contributor.authorHawi, Z.
dc.contributor.authorPang, K.C.
dc.contributor.authorParish, C.L.
dc.contributor.authorPolo, J.M.
dc.contributor.authorBellgrove, M.A.
dc.date.issued2019
dc.description.abstractPeripheral blood mononuclear cells were donated by a male teenager with clinically diagnosed attention deficit hyperactivity disorder (ADHD) under the Diagnostic and Statistical Manual of Mental Disorders IV criteria and his unaffected male sibling. Induced pluripotent stem cells were developed using integration-free Sendai Reprogramming factors containing OCT4, SOX2, KLF4, and c-MYC. All four iPSC lines displayed pluripotent cell morphology, pluripotency-associated factors at the DNA and protein level, alkaline phosphatase enzymatic activity and a male karyotype of 46, XY. All lines had capacity for in vitro differentiation into all the three germ layers. All were negative for Mycoplasma.
dc.description.statementofresponsibilityJanette Tong, Kyung Min Leea, Xiaodong Liu, Christian M.Nefzger, Prasidhee Vijayakumar, Ziarih Hawi ... et al.
dc.identifier.citationStem Cell Research, 2019; 34:101353-101353
dc.identifier.doi10.1016/j.scr.2018.11.014
dc.identifier.issn1873-5061
dc.identifier.issn1876-7753
dc.identifier.orcidPolo, J.M. [0000-0002-2531-778X]
dc.identifier.urihttps://hdl.handle.net/2440/133465
dc.language.isoen
dc.publisherElsevier
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1112452
dc.rights© 2018 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/BY-NC-ND/4.0
dc.source.urihttps://doi.org/10.1016/j.scr.2018.11.014
dc.subjectLeukocytes, Mononuclear
dc.subjectCell Line
dc.subjectHumans
dc.subjectCell Culture Techniques
dc.subjectFamily
dc.subjectSiblings
dc.subjectAttention Deficit Disorder with Hyperactivity
dc.subjectAdolescent
dc.subjectAustralia
dc.subjectMale
dc.subjectInduced Pluripotent Stem Cells
dc.subjectKruppel-Like Factor 4
dc.subjectWhite People
dc.subject.meshLeukocytes, Mononuclear
dc.subject.meshCell Line
dc.subject.meshHumans
dc.subject.meshCell Culture Techniques
dc.subject.meshFamily
dc.subject.meshSiblings
dc.subject.meshAttention Deficit Disorder with Hyperactivity
dc.subject.meshAdolescent
dc.subject.meshEuropean Continental Ancestry Group
dc.subject.meshAustralia
dc.subject.meshMale
dc.subject.meshInduced Pluripotent Stem Cells
dc.titleGeneration of four iPSC lines from peripheral blood mononuclear cells (PBMCs) of an attention deficit hyperactivity disorder (ADHD) individual and a healthy sibling in an Australia-Caucasian family
dc.typeJournal article
pubs.publication-statusPublished

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
hdl_133465.pdf
Size:
919.71 KB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Medical Sciences publications