Measuring the interaction forces between protein inclusion bodies and an air bubble using an atomic force microscope

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dc.contributor.authorWangsaWirawan, N.
dc.contributor.authorIkai, A.
dc.contributor.authorO'Neill, B.
dc.contributor.authorMiddelberg, A.
dc.date.issued2001
dc.descriptionCopyright © 2001 American Chemical Society and American Institute of Chemical Engineers
dc.description.abstractInteraction forces between protein inclusion bodies and an air bubble have been quantified using an atomic force microscope (AFM). The inclusion bodies were attached to the AFM tip by covalent bonds. Interaction forces measured in various buffer concentrations varied from 9.7 nN to 25.3 nN (( 4-11%) depending on pH. Hydrophobic forces provide a stronger contribution to overall interaction force than electrostatic double layer forces. It also appears that the ionic strength affects the interaction force in a complex way that cannot be directly predicted by DLVO theory. The effects of pH are significantly stronger for the inclusion body compared to the air bubble. This study provides fundamental information that will subsequently facilitate the rational design of flotation recovery system for inclusion bodies. It has also demonstrated the potential of AFM to facilitate the design of such processes from a practical viewpoint.
dc.description.statementofresponsibilityN. D. Wangsa-Wirawan, A. Ikai, B. K. O’Neill and A. P. J. Middelberg
dc.identifier.citationBiotechnology Progress, 2001; 17(5):963-969
dc.identifier.doi10.1021/bp010072
dc.identifier.issn8756-7938
dc.identifier.issn1520-6033
dc.identifier.urihttp://hdl.handle.net/2440/793
dc.language.isoen
dc.publisherAmer Chemical Soc
dc.source.urihttp://www3.interscience.wiley.com/cgi-bin/fulltext/121399876/PDFSTART
dc.subjectInclusion Bodies
dc.subjectHumans
dc.subjectEscherichia coli
dc.subjectInsulin-Like Growth Factor I
dc.subjectRecombinant Proteins
dc.subjectBuffers
dc.subjectMicroscopy, Atomic Force
dc.subjectSpecimen Handling
dc.subjectAir
dc.subjectHydrogen-Ion Concentration
dc.subjectOsmolar Concentration
dc.subjectStatic Electricity
dc.titleMeasuring the interaction forces between protein inclusion bodies and an air bubble using an atomic force microscope
dc.typeJournal article
pubs.publication-statusPublished

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