A molecular and evolutionary study of the β-globin gene family of the Australian marsupial Sminthopsis Crassicaudata

Simple item page
dc.contributor.authorCooper, S.
dc.contributor.authorMurphy, R.
dc.contributor.authorDolman, G.
dc.contributor.authorHussey, D.
dc.contributor.authorHope, R.
dc.date.issued1996
dc.description.abstractBeta-globin gene families in eutherians (placental mammals) consist of a set of four or more developmentally regulated genes which are closely linked and, in general, arranged in the order 5'-embryonic/fetal genes-adult genes-3'. This cluster of genes is proposed to have arisen by tandem duplication of ancestral beta-globin genes, with the first duplication occurring 200 to 155 MYBP just prior to a period in mammalian evolution when eutherians and marsupials diverged from a common ancestor. In this paper we trace the evolutionary history of the beta-globin gene family back to the origins of these mammals by molecular characterization of the beta-globin gene family of the Australian marsupial Sminthopsis crassicaudata. Using Southern and restriction analysis of total genomic DNA and bacteriophage clones of beta-like globin genes, we provide evidence that just two functional beta-like globin genes exist in this marsupial, including one embryonic-expressed gene (S.c-epsilon) and one adult-expressed gene (S.c-beta), linked in the order 5'-epsilon-beta-3'. The entire DNA sequence of the adult beta-globin gene is reported and shown to be orthologous to the adult beta-globin genes of the North American marsupial Didelphis virginiana and eutherian mammals. These results, together with results from a phylogenetic analysis of mammalian beta-like globin genes, confirm the hypothesis that a two-gene cluster, containing an embryonic- and an adult-expressed beta-like globin gene, existed in the most recent common ancester of marsupials and eutherians. Northern analysis of total RNA isolated from embryos and neonatals indicates that a switch from embryonic to adult gene expression occurs at the time of birth, coinciding with the transfer of the marsupial from a uterus to a pouch environment.
dc.description.statementofresponsibilityS J Cooper, R Murphy, G Dolman, D Hussey and R M Hope
dc.identifier.citationMolecular Biology and Evolution (MBE), 1996; 13(7):1012-1022
dc.identifier.doi10.1093/oxfordjournals.molbev.a025651
dc.identifier.issn0737-4038
dc.identifier.issn1537-1719
dc.identifier.orcidCooper, S. [0000-0002-7843-8438]
dc.identifier.orcidDolman, G. [0000-0001-7611-6841]
dc.identifier.urihttp://hdl.handle.net/2440/11481
dc.language.isoen
dc.publisherSOC MOLECULAR BIOLOGY EVOLUTION
dc.rights© 1996 Society for Molecular Biology and Evolution
dc.source.urihttp://mbe.oxfordjournals.org.proxy.library.adelaide.edu.au/content/13/7/1012.abstract
dc.subjectAnimals
dc.subjectMarsupialia
dc.subjectDeoxyribonuclease BamHI
dc.subjectSite-Specific DNA-Methyltransferase (Adenine-Specific)
dc.subjectGlobins
dc.subjectDNA Probes
dc.subjectBlotting, Northern
dc.subjectBlotting, Southern
dc.subjectIn Situ Hybridization
dc.subjectRestriction Mapping
dc.subjectCloning, Molecular
dc.subjectAge Factors
dc.subjectEvolution, Molecular
dc.subjectPhylogeny
dc.subjectGene Expression Regulation, Developmental
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectMultigene Family
dc.subjectMolecular Sequence Data
dc.subjectAustralia
dc.subjectGenetic Variation
dc.subjectGenetic Linkage
dc.titleA molecular and evolutionary study of the β-globin gene family of the Australian marsupial Sminthopsis Crassicaudata
dc.title.alternativeA molecular and evolutionary study of the beta-globin gene family of the Australian marsupial Sminthopsis crassicaudata
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Genetics publications
Environment Institute Leaders publications
Environment Institute publications