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A custom-made device for reproducibly depositing pre-metered doses of nebulized drugs on pulmonary cells in vitro

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dc.contributor.authorHorstmann, J.C.
dc.contributor.authorThorn, C.R.
dc.contributor.authorCarius, P.
dc.contributor.authorGraef, F.
dc.contributor.authorMurgia, X.
dc.contributor.authorde Souza Carvalho Wodarz, C.
dc.contributor.authorLehr, C.M.
dc.date.issued2021
dc.descriptionData source: Supplementary data, https://doi.org/10.3389/fbioe.2021.643491
dc.description.abstractThe deposition of pre-metered doses (i.e., defined before and not after exposition) at the air–liquid interface of viable pulmonary epithelial cells remains an important but challenging task for developing aerosol medicines. While some devices allow quantification of the deposited dose after or during the experiment, e.g., gravimetrically, there is still no generally accepted way to deposit small pre-metered doses of aerosolized drugs or pharmaceutical formulations, e.g., nanomedicines. Here, we describe a straightforward custom-made device, allowing connection to commercially available nebulizers with standard cell culture plates. Designed to tightly fit into the approximately 12-mm opening of either a 12-well Transwell® insert or a single 24-well plate, a defined dose of an aerosolized liquid can be directly deposited precisely and reproducibly (4.8% deviation) at the air–liquid interface (ALI) of pulmonary cell cultures. The deposited dose can be controlled by the volume of the nebulized solution, which may vary in a range from 20 to 200 μl. The entire nebulization-deposition maneuver is completed after 30 s and is spatially homogenous. After phosphate-buffered saline (PBS) deposition, the viability and barrier properties transepithelial electrical resistance (TEER) of human bronchial epithelial Calu-3 cells were not negatively affected. Straightforward in manufacture and use, the device enables reproducible deposition of metered doses of aerosolized drugs to study the interactions with pulmonary cell cultures grown at ALI conditions
dc.identifier.citationFrontiers in Bioengineering and Biotechnology, 2021; 9(643491):1-12
dc.identifier.doi10.3389/fbioe.2021.643491
dc.identifier.issn2296-4185
dc.identifier.issn2296-4185
dc.identifier.urihttps://hdl.handle.net/11541.2/25613
dc.language.isoen
dc.publisherFrontiers Research Foundation
dc.rightsCopyright 2021 The author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. (https://creativecommons.org/licenses/by/4.0/)
dc.source.urihttps://doi.org/10.3389/fbioe.2021.643491
dc.subjectinhalation
dc.subjectaerosol
dc.subjectpulmonary drug delivery
dc.subjectepithelial cells
dc.subjectair–liquid interface
dc.titleA custom-made device for reproducibly depositing pre-metered doses of nebulized drugs on pulmonary cells in vitro
dc.typeJournal article
pubs.publication-statusPublished
ror.fileinfo12233185230001831 13233185220001831 fbioe-09-643491
ror.mmsid9916524505101831

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