Current studentsExisting staffContact us
 

Infertility caused by hCG autoantibody

Simple item page
dc.contributor.authorAmato, F.
dc.contributor.authorWarnes, G.
dc.contributor.authorKirby, C.
dc.contributor.authorNorman, R.
dc.date.issued2002
dc.descriptionCopyright © 2002 by The Endocrine Society
dc.description.abstractAn anti-hCG autoantibody was found in a patient with a 9-yr history of secondary infertility. Although the patient had regular menstrual cycles, had conceived spontaneously, and had good hormonal and follicular responses to gonadotropic stimulation regimens during the in vitro fertilization work-up, she presented with apparent recurrent pregnancy loss associated with prolonged raised hCG levels. Initially the presence of a high mol wt hCG complex was demonstrated in the serum by gel chromatography. The binding of [125I]recombinant hCG to a serum sample and subsequently to the affinity-purified IgG from the same sample revealed the presence of an hCG antibody. The antiserum was shown to be specific, with a low affinity (Ka, 1.4 x 106 liters/mol), but a high capacity (418 nmol/liter), for hCG. Cross-reaction with recombinant human FSH, recombinant human LH, hCG, and hCGß were low (<0.019%, 0.021%, 0.039%, and 0.006%, respectively). In addition, heat-inactivated serum and the affinity-purified IgG were shown to inhibit the action of hCG in an in vitro bioassay. We suggest that the persisting titer of the antibody to be responsible for the patient’s infertility.
dc.description.statementofresponsibilityFred Amato, Graham M. Warnes, Christine A. Kirby and Robert J. Norman
dc.identifier.citationJournal of Clinical Endocrinology and Metabolism (JCEM), 2002; 87(3):993-997
dc.identifier.doi10.1210/jc.87.3.993
dc.identifier.issn0021-972X
dc.identifier.issn1945-7197
dc.identifier.orcidNorman, R. [0000-0002-3118-3896]
dc.identifier.urihttp://hdl.handle.net/2440/8265
dc.language.isoen
dc.publisherEndocrine Society
dc.source.urihttps://doi.org/10.1210/jc.87.3.993
dc.subjectLeydig Cells
dc.subjectCell Line, Transformed
dc.subjectHumans
dc.subjectInfertility, Female
dc.subjectProgesterone
dc.subjectChorionic Gonadotropin
dc.subjectImmunoglobulin G
dc.subjectRecombinant Proteins
dc.subjectAutoantibodies
dc.subjectAntibody Specificity
dc.subjectAdolescent
dc.subjectFemale
dc.subjectMale
dc.subjectBlood Physiological Phenomena
dc.titleInfertility caused by hCG autoantibody
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Obstetrics and Gynaecology publications