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The formin protein RMD regulates rice stomatal opening by modulating tonoplast-endoplasmic reticulum associations

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dc.contributor.authorLiu, Z.
dc.contributor.authorChang, S.
dc.contributor.authorYong, M.
dc.contributor.authorOsterlund, I.
dc.contributor.authorQu, Z.
dc.contributor.authorRao, J.
dc.contributor.authorCai, W.
dc.contributor.authorZhang, J.
dc.contributor.authorCao, L.
dc.contributor.authorShi, J.
dc.contributor.authorChen, Z.-H.
dc.contributor.authorPersson, S.
dc.contributor.authorLiang, W.
dc.contributor.authorZhang, D.
dc.date.issued2025
dc.description.abstractThe stoma is key to plant growth and development, with highly controlled functions. However, the regulatory mechanisms of stomata remain largely unknown in monocot crop species. In this study, we reveal that guard cell physiology is regulated by actin cytoskeleton-mediated changes to tonoplast-endoplasmic reticulum (ER) contact sites in rice. This mechanism is mediated via the formin protein Rice Morphology Determinant and the vesicle-associated membrane protein-associated protein VAP1 at intersections of the ER and actin filaments. Notably, monocot (rice) guard cells contain two distinct types of vacuoles, where the central vacuole size and stomatal aperture are regulated by the actin cytoskeleton. Furthermore, VAP1 localized at both the ER and central vacuole, which closely aligned with the ER and actin cytoskeleton. Genetic interactions of vap1 and rmd-1 led to defects in rice stomatal aperture, which impacted light-induced stomatal opening. Our results significantly extend our knowledge of crop stomatal regulation.
dc.description.statementofresponsibilityZengyu Liu, Shuwei Chang, Miingtiem Yong, Isabella Østerlund, Zhuo Qu, Jinliang Rao, Wenguo Cai, Jiao Zhang, Lingyan Cao, Jianxin Shi, Zhong-Hua Chen, Staffan Persson, Wanqi Liang, and Dabing Zhang
dc.identifier.citationCell reports, 2025; 44(12):116689-1-116689-20
dc.identifier.doi10.1016/j.celrep.2025.116689
dc.identifier.issn2211-1247
dc.identifier.issn2211-1247
dc.identifier.orcidChen, Z.-H. [0000-0002-7531-320X]
dc.identifier.urihttps://hdl.handle.net/2440/148496
dc.language.isoen
dc.publisherElsevier
dc.relation.granthttp://purl.org/au-research/grants/arc/FT210100366
dc.rights© 2025 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.source.urihttps://doi.org/10.1016/j.celrep.2025.116689
dc.subjectactin cytoskeleton
dc.subjectCP: cell biology
dc.subjectCP: plants
dc.subjectendoplasmic reticulum
dc.subjectformin
dc.subjectrice
dc.subjectstomatal opening
dc.subjectvacuole
dc.titleThe formin protein RMD regulates rice stomatal opening by modulating tonoplast-endoplasmic reticulum associations
dc.typeJournal article
pubs.publication-statusPublished

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