Somatostatin receptor subtype-4 agonist NNC 26-9100 mitigates the effect of soluble Aβ₄₂ oligomers via a metalloproteinase-dependent mechanism

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dc.contributor.authorSandoval, K.
dc.contributor.authorFarr, S.
dc.contributor.authorBanks, W.
dc.contributor.authorCrider, A.
dc.contributor.authorMorley, J.
dc.contributor.authorWitt, K.
dc.date.issued2013
dc.description.abstractSoluble amyloid-β peptide (Aβ) oligomers have been hypothesized to be primary mediators of Alzheimer's disease progression. In this regard, reduction of soluble Aβ-oligomers levels within the brain may provide a viable means in which to treat the disease. Somatostatin receptor subtype-4 (SSTR4) agonists have been proposed to reduce Aβ levels in the brain via enhancement of enzymatic degradation. Herein we evaluated the effect of selective SSTR4 agonist NNC 26-9100 on the changes in learning and soluble Aβ42 oligomer brain content with and without co-administration of the M13-metalloproteinase family enzyme-inhibitor phosphoramidon, using the senescence-accelerated mouse prone-8 (SAMP8) model. NNC 26-9100 treatment (0.2 µg i.c.v. in 2 µL) improved learning, which was blocked by phosphoramidon (1 and 10mM, respectively). NNC 26-9100 decreased total soluble Aβ42, an effect which was blocked by phosphoramidon (10mM). Extracellular, intracellular, and membrane fractions were then isolated from cortical tissue and assessed for soluble oligomer alterations. NNC 26-9100 decreased the Aβ42 trimeric (12 kDa) form within the extracellular and intracellular fractions, and produced a band-split effect of the Aβ42 hexameric (25 kDa) form within the extracellular fraction. These effects were also blocked by phosphoramdon (1 and 10mM, respectively). Subsequent evaluation of NNC 26-9100 in APPswe Tg2576 transgenic mice showed a similar learning improvement and corresponding reduction in soluble Aβ42 oligomers within extracellular, intracellular, and membrane fractions. These data support the hypothesis that NNC 26-9100 reduces soluble Aβ42 oligomers and enhances learning through a phosphoramidon-sensitive metalloproteinase-dependent mechanism.
dc.description.statementofresponsibilityKarin E. Sandoval, Susan A. Farr, William A. Banks, Albert M. Crider, John E. Morley, Ken A. Witt
dc.identifier.citationBrain Research, 2013; 1520:145-156
dc.identifier.doi10.1016/j.brainres.2013.05.006
dc.identifier.issn0006-8993
dc.identifier.issn1872-6240
dc.identifier.orcidMorley, J. [0000-0001-6444-2965]
dc.identifier.urihttp://hdl.handle.net/2440/85807
dc.language.isoen
dc.publisherElsevier Science
dc.rights© 2013 Elsevier B.V. All rights reserved.
dc.source.urihttps://doi.org/10.1016/j.brainres.2013.05.006
dc.subjectPhosphoramidon; NNC 26-9100; somatostatin receptor subtype-4; amyloid-beta oligomer
dc.titleSomatostatin receptor subtype-4 agonist NNC 26-9100 mitigates the effect of soluble Aβ₄₂ oligomers via a metalloproteinase-dependent mechanism
dc.title.alternativeSomatostatin receptor subtype-4 agonist NNC 26-9100 mitigates the effect of soluble Abeta42 oligomers via a metalloproteinase-dependent mechanism
dc.typeJournal article
pubs.publication-statusPublished

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