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The identification of the transcriptional regulator CRP in Aeromonas hydrophila JMP636 and its involvement in amylase production and the 'acidic toxicity' effect

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dc.contributor.authorKidd, S.P.
dc.contributor.authorPemberton, J.M.
dc.date.issued2002
dc.description.abstractAims: The physiological examination of amylase production by Aeromonas hydrophila JMP636 and identification of the mechanism of regulation. Methods and Results:Aeromonas hydrophila JMP636 was grown with single, then dual carbon sources; the growth cycle was followed and amylase activity throughout was monitored. The levels of cAMP, a known secondary messenger for the regulatory gene crp, were also examined. Amylase activity was regulated by catabolite repression. Physiological studies revealed that JMP636 exhibited both diauxic growth, with two carbon sources, and the ‘acid toxicity’ effect on glucose. The crp gene was cloned, expressed and inactivated from the JMP636 chromosome. Catabolite repression of amylase production and the ‘acid toxicity’ effect both require crp and were linked to cAMP levels. Conclusions: Regulation of amylase production was predicted to follow the model CRP-mediated cAMP-dependent Escherichia coli catabolite regulation system. Significance and Impact of the Study: This work provides an understanding of the physiology of the opportunistic pathogen Aer. hydrophila through identification of the mechanism of catabolite repression of amylase production and the existence of crp within this cell. It also provides a broader knowledge of global gene regulation and suggests regulatory mechanisms of other Aer. hydrophila gene/s.
dc.description.statementofresponsibilityS.P. Kidd, J.M. Pemberton
dc.identifier.citationJournal of Applied Microbiology, 2002; 93(5):787-793
dc.identifier.doi10.1046/j.1365-2672.2002.01750.x
dc.identifier.issn1364-5072
dc.identifier.issn1365-2672
dc.identifier.orcidKidd, S.P. [0000-0002-2118-1651]
dc.identifier.urihttp://hdl.handle.net/2440/97784
dc.language.isoen
dc.publisherWiley
dc.rights© 2002 The Society for Applied Microbiology
dc.source.urihttps://doi.org/10.1046/j.1365-2672.2002.01750.x
dc.subjectAeromonas hydrophila
dc.subjectAmylases
dc.subjectGlucose
dc.subjectCyclic AMP Receptor Protein
dc.subjectCyclic AMP
dc.subjectGenes, Regulator
dc.subjectHydrogen-Ion Concentration
dc.subjectCarbohydrate Metabolism
dc.titleThe identification of the transcriptional regulator CRP in Aeromonas hydrophila JMP636 and its involvement in amylase production and the 'acidic toxicity' effect
dc.typeJournal article
pubs.publication-statusPublished

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