Molecular cloning of cDNA encoding a novel platelet-endothelial cell tetra-span antigen, PETA-3

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dc.contributor.authorFitter, S.
dc.contributor.authorTetaz, T.J.
dc.contributor.authorBerndt, M.C.
dc.contributor.authorAshman, L.K.
dc.date.issued1995
dc.description.abstractPlatelet-endothelial cell tetra-span antigen (PETA-3) was originally identified as a novel human platelet surface glycoprotein, gp27, which was detected by a monoclonal antibody (MoAb), 14A2.H1. Although this glycoprotein is present in low abundance on the platelet surface, MoAb 14A2.H1 stimulates platelet aggregation and mediator release. We now report isolation of a cDNA clone encoding PETA-3 from a library derived from the megakaryoblastic leukemia cell line MO7e. The clone encodes an open reading frame of 253 amino acids that displays 25% to 30% amino acid sequence identity with several members of the newly defined Tetraspan, or Transmembrane 4 superfamily. These proteins consist of four conserved putative transmembrane domains with a large divergent extracellular loop between the third and fourth membrane-spanning regions. PETA-3 has a single consensus sequence for N-linked glycosylation located in this extracellular loop. A single PETA-3 RNA transcript (1.6 kb) was detected in RNA isolated from MO7e cells, bone marrow stromal cells, the C11 endothelial cell line, and several myeloid leukemia cell lines. No transcript was detected in the lymphoblastoid cell lines MOLT-4 and BALM-1. This pattern correlates well with previous protein expression data. Northern blot analysis of RNA from a range of human tissues indicated that the transcript was present in most tissues, the notable exception being brain.
dc.description.statementofresponsibilityStephen Fitter, Tim J. Tetaz, Michael C. Berndt, and Leonie K. Ashman
dc.identifier.citationBlood, 1995; 86(4):1348-1355
dc.identifier.doi10.1182/blood.v86.4.1348.bloodjournal8641348
dc.identifier.issn0006-4971
dc.identifier.issn1528-0020
dc.identifier.orcidFitter, S. [0000-0003-1663-6807]
dc.identifier.urihttp://hdl.handle.net/2440/103301
dc.language.isoen
dc.publisherAmerican Society of Hematology
dc.rights© 1995 by The American Society of Hematology.
dc.source.urihttp://www.bloodjournal.org.proxy.library.adelaide.edu.au/content/86/4/1348
dc.subjectBlood Platelets
dc.subjectHumans
dc.subjectPlatelet Membrane Glycoproteins
dc.subjectDNA, Complementary
dc.subjectRNA, Messenger
dc.subjectDNA Primers
dc.subjectAntigens, CD
dc.subjectCloning, Molecular
dc.subjectSequence Alignment
dc.subjectGene Expression
dc.subjectAmino Acid Sequence
dc.subjectBase Sequence
dc.subjectSequence Homology, Amino Acid
dc.subjectSolubility
dc.subjectMolecular Sequence Data
dc.subjectTetraspanin 24
dc.titleMolecular cloning of cDNA encoding a novel platelet-endothelial cell tetra-span antigen, PETA-3
dc.typeJournal article
pubs.publication-statusPublished

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