Fructilactobacillus cliffordii sp. nov., Fructilactobacillus hinvesii sp. nov., Fructilactobacillus myrtifloralis sp. nov., Fructilactobacillus carniphilus sp. nov. and Fructobacillus americanaquae sp. nov., five novel lactic acid bacteria isolated from insects or flowers of Kangaroo Island, South Australia

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dc.contributor.authorOliphant, S.A.
dc.contributor.authorWatson-Haigh, N.S.
dc.contributor.authorSumby, K.M.
dc.contributor.authorGardner, J.M.
dc.contributor.authorJiranek, V.
dc.date.issued2023
dc.description.abstractSix strains, KI11_D11T, KI4_B1, KI11_C11T, KI16_H9T, KI4_A6T and KI3_B9T, were isolated from insects and flowers on Kangaroo Island, South Australia. On the basis of 16S rRNA gene phylogeny, strains KI11_D11T, KI4_B1, KI11_C11T, KI16_H9T, KI4_A6T were found to be closely related to Fructilactobacillus ixorae Ru20-1T. Due to the lack of a whole genome sequence for this species, whole genome sequencing of Fructilactobacillus ixorae Ru20-1T was undertaken. KI3_B9T was found to be closely related to Fructobacillus tropaeoli F214-1T. Utilizing core gene phylogenetics and whole genome analyses, such as determination of AAI, ANI and dDDH, we propose that these six isolates represent five novel species with the names Fructilactobacillus cliffordii (KI11_D11T= LMG 32130T = NBRC 114988T), Fructilactobacillus hinvesii (KI11_C11T = LMG 32129T = NBRC 114987T), Fructilactobacillus myrtifloralis (KI16_H9T= LMG 32131T = NBRC 114989T) Fructilactobacillus carniphilus (KI4_A6T = LMG 32127T = NBRC 114985T) and Fructobacillus americanaquae (KI3_B9T = LMG 32124T = NBRC 114983T). Chemotaxonomic analyses detected no fructophilic characters for these strains of member of the genus Fructilactobacillus. KI3_B9T was found to be obligately fructophilic, similarly to its phylogenetic neighbours in the genus Fructobacillus. This study represents the first isolation, to our knowledge, of novel species in the family Lactobacillaceae from the Australian wild.
dc.description.statementofresponsibilityScott A. Oliphant, Nathan S. Watson-Haigh, Krista M. Sumby, Jennifer M. Gardner and Vladimir Jiranek
dc.identifier.citationInternational Journal of Systematic and Evolutionary Microbiology, 2023; 73(2):005730-1-005730-14
dc.identifier.doi10.1099/ijsem.0.005730
dc.identifier.issn1466-5026
dc.identifier.issn1466-5034
dc.identifier.orcidOliphant, S.A. [0000-0001-5133-0198]
dc.identifier.orcidSumby, K.M. [0000-0002-1833-2025]
dc.identifier.orcidGardner, J.M. [0000-0002-4215-955X]
dc.identifier.orcidJiranek, V. [0000-0002-9775-8963]
dc.identifier.urihttps://hdl.handle.net/2440/137619
dc.language.isoen
dc.publisherMicrobiology Society
dc.relation.granthttp://purl.org/au-research/grants/arc/IC70100008
dc.rights© 2023 The Authors. This is an open-access article distributed under the terms of the Creative Commons Attribution License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
dc.source.urihttps://doi.org/10.1099/ijsem.0.005730
dc.subjectApis mellifera; Australian lactic acid bacteria; Blatta orientalis; Calytrix glaberrima; European honey bees; FLAB; Fructobacillus; fructophilic lactic acid bacteria; Fructilactobacillus; Kangaroo Island; Melaleuca gibbosa; meat ant; Iridomyrmex purpureus; oriental cockroach; smooth fringe-myrtle; slender honey-myrtle
dc.subject.meshAnimals
dc.subject.meshLactobacillus
dc.subject.meshFlowers
dc.subject.meshFatty Acids
dc.subject.meshDNA, Bacterial
dc.subject.meshRNA, Ribosomal, 16S
dc.subject.meshBacterial Typing Techniques
dc.subject.meshSequence Analysis, DNA
dc.subject.meshPhylogeny
dc.subject.meshBase Composition
dc.subject.meshAustralia
dc.subject.meshSouth Australia
dc.subject.meshLactobacillales
dc.subject.meshInsecta
dc.titleFructilactobacillus cliffordii sp. nov., Fructilactobacillus hinvesii sp. nov., Fructilactobacillus myrtifloralis sp. nov., Fructilactobacillus carniphilus sp. nov. and Fructobacillus americanaquae sp. nov., five novel lactic acid bacteria isolated from insects or flowers of Kangaroo Island, South Australia
dc.typeJournal article
pubs.publication-statusPublished

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