An improved reprogrammable mouse model harbouring the reverse tetracycline-controlled transcriptional transactivator 3

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dc.contributor.authorAlaei, S.
dc.contributor.authorKnaupp, A.S.
dc.contributor.authorLim, S.M.
dc.contributor.authorChen, J.
dc.contributor.authorHolmes, M.L.
dc.contributor.authorÄnkö, M.L.
dc.contributor.authorNefzger, C.M.
dc.contributor.authorPolo, J.M.
dc.date.issued2016
dc.description.abstractReprogrammable mouse models engineered to conditionally express Oct-4, Klf-4, Sox-2 and c-Myc (OKSM) have been instrumental in dissecting molecular events underpinning the generation of induced pluripotent stem cells. However, until now these models have been reported in the context of the m2 reverse tetracycline-controlled transactivator, which results in low reprogramming efficiency and consequently limits the number of reprogramming intermediates that can be isolated for downstream profiling. Here, we describe an improved OKSM mouse model in the context of the reverse tetracycline-controlled transactivator 3 with enhanced reprogramming efficiency (>9-fold) and increased numbers of reprogramming intermediate cells albeit with similar kinetics, which we believe will facilitate mechanistic studies of the reprogramming process.
dc.description.statementofresponsibilityS. Alaei, A.S. Knaupp, S.M.Lim, J.Chen, M.L.Holmes, M.L.Änköa
dc.identifier.citationStem Cell Research, 2016; 17(1):49-53
dc.identifier.doi10.1016/j.scr.2016.05.008
dc.identifier.issn1873-5061
dc.identifier.issn1876-7753
dc.identifier.orcidPolo, J.M. [0000-0002-2531-778X]
dc.identifier.urihttps://hdl.handle.net/2440/133479
dc.language.isoen
dc.publisherElsevier
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1092280
dc.relation.granthttp://purl.org/au-research/grants/nhmrc/1085302
dc.rights© 2016 The Author(s). Published by Elsevier B.V.
dc.source.urihttps://doi.org/10.1016/j.scr.2016.05.008
dc.subjectCells, Cultured
dc.subjectFibroblasts
dc.subjectAnimals
dc.subjectMice
dc.subjectTeratoma
dc.subjectTetracyclines
dc.subjectProto-Oncogene Proteins c-myc
dc.subjectCell Differentiation
dc.subjectPlasmids
dc.subjectOctamer Transcription Factor-3
dc.subjectKruppel-Like Transcription Factors
dc.subjectTranscriptional Activation
dc.subjectSOXB1 Transcription Factors
dc.subjectInduced Pluripotent Stem Cells
dc.subjectCellular Reprogramming
dc.subject.meshCells, Cultured
dc.subject.meshFibroblasts
dc.subject.meshAnimals
dc.subject.meshMice
dc.subject.meshTeratoma
dc.subject.meshTetracyclines
dc.subject.meshProto-Oncogene Proteins c-myc
dc.subject.meshCell Differentiation
dc.subject.meshPlasmids
dc.subject.meshOctamer Transcription Factor-3
dc.subject.meshKruppel-Like Transcription Factors
dc.subject.meshTranscriptional Activation
dc.subject.meshSOXB1 Transcription Factors
dc.subject.meshInduced Pluripotent Stem Cells
dc.subject.meshCellular Reprogramming
dc.titleAn improved reprogrammable mouse model harbouring the reverse tetracycline-controlled transcriptional transactivator 3
dc.typeJournal article
pubs.publication-statusPublished

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