High-resolution genotyping of wild barley introgression lines and fine-mapping of the threshability locus thresh-1 using the Illumina GoldenGate assay

Simple item page
dc.contributor.authorSchmalenbach, I.
dc.contributor.authorMarch, T.
dc.contributor.authorBringezu, T.
dc.contributor.authorWaugh, R.
dc.contributor.authorPillen, K.
dc.date.issued2011
dc.description.abstractGenetically well-characterized mapping populations are a key tool for rapid and precise localization of quantitative trait loci (QTL) and subsequent identification of the underlying genes. In this study, a set of 73 introgression lines (S42ILs) originating from a cross between the spring barley cultivar Scarlett (Hordeum vulgare ssp. vulgare) and the wild barley accession ISR42-8 (H. v. ssp. spontaneum) was subjected to high-resolution genotyping with an Illumina 1536-SNP array. The array enabled a precise localization of the wild barley introgressions in the elite barley background. Based on 636 informative SNPs, the S42IL set represents 87.3% of the wild barley genome, where each line contains on average 3.3% of the donor genome. Furthermore, segregating high-resolution mapping populations (S42IL-HRs) were developed for 70 S42ILs in order to facilitate QTL fine-mapping and cloning. As a case study, we used the developed genetic resources to rapidly identify and fine-map the novel locus thresh-1 on chromosome 1H that controls grain threshability. Here, the recessive wild barley allele confers a difficult to thresh phenotype, suggesting that thresh-1 played an important role during barley domestication. Using a S42IL-HR population, thresh-1 was fine-mapped within a 4.3cM interval that was predicted to contain candidate genes involved in regulation of plant cell wall composition. The set of wild barley introgression lines and derived high-resolution populations are ideal tools to speed up the process of mapping and further dissecting QTL, which ultimately clears the way for isolating the genes behind QTL effects.
dc.description.statementofresponsibilityInga Schmalenbach, Timothy J. March, Thomas Bringezu, Robbie Waugh and Klaus Pillen
dc.identifier.citationG3: Genes, Genomes, Genetics, 2011; 1(3):187-196
dc.identifier.doi10.1534/g3.111.000182
dc.identifier.issn2160-1836
dc.identifier.issn2160-1836
dc.identifier.urihttp://hdl.handle.net/2440/71584
dc.language.isoen
dc.publisherGenetics Society of America
dc.rightsCopyright © 2011 Schmalenbach et al. This is an open access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttps://doi.org/10.1534/g3.111.000182
dc.subjectWild barley introgression lines
dc.subjectIllumina GoldenGate assay
dc.subjecthigh-resolution mapping populations
dc.subjectthreshability locus thresh-1
dc.titleHigh-resolution genotyping of wild barley introgression lines and fine-mapping of the threshability locus thresh-1 using the Illumina GoldenGate assay
dc.typeJournal article
pubs.publication-statusPublished

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
hdl_71584.pdf
Size:
1.28 MB
Format:
Adobe Portable Document Format
Description:
Published version
Download

Collections

Agriculture, Food and Wine publications