Finding proteins involved with black point of barley using proteomics
Date
2005
Authors
March, T.
Schultz, C.
Able, J.
Able, A.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Conference paper
Citation
12th Australian Barley Technical Symposium Conference Proceedings, 11-14 September 2005: 4p. [CD-ROM]
Statement of Responsibility
Conference Name
Australian Barley Technical Symposium (12th : 2005 : Hobart, Tasmania)
Abstract
Black point of barley refers to the discolouration of the embryo end of the grain. Downgrading of malting barley to feed grade due to black point results in significant economic loss to the Australian barley industry. Discolouration appears to be a result of enzymatic oxidation of phenolic compounds within the grain with several biochemical pathways involved. To find individual proteins involved in black point formation we are using a broad-based proteomics approach. Using 2D gel electrophoresis, expression profiles of water-soluble and cell wall proteins from black pointed and healthy husk tissue have been compared. Identification of unique proteins involved with black point formation or susceptibility will aid in developing new germplasm with increased tolerance to black point.