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Rice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4

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dc.contributor.authorZhu, W.
dc.contributor.authorYang, L.
dc.contributor.authorWu, D.
dc.contributor.authorMeng, Q.
dc.contributor.authorDeng, X.
dc.contributor.authorHuang, G.
dc.contributor.authorZhang, J.
dc.contributor.authorChen, X.
dc.contributor.authorFerrándiz, C.
dc.contributor.authorLiang, W.
dc.contributor.authorDreni, L.
dc.contributor.authorZhang, D.
dc.date.issued2022
dc.descriptionFirst published: 12 November 2021
dc.description.abstractThe spatiotemporal control of meristem identity is critical for determining inflorescence architecture, and thus yield, of cereal plants. However, the precise mechanisms underlying inflorescence and spikelet meristem determinacy in cereals are still largely unclear. We have generated loss-of-function and overexpression mutants of the paralogous OsMADS5 and OsMADS34 genes in rice (Oryza sativa), and analysed their panicle phenotypes. Using chromatin immunoprecipitation, electrophoretic mobility-shift and dual-luciferase assays, we have also identified RICE CENTRORADIALIS 4 (RCN4), a TFL1-like gene, as a direct downstream target of both OsMADS proteins, and have analysed RCN4 mutants. The osmads5 osmads34 mutant lines had significantly enhanced panicle branching with increased secondary, and even tertiary and quaternary, branches, compared to wild type and osmads34 plants. The osmads34 mutant phenotype could largely be rescued by also knocking out RCN4. Moreover, transgenic panicles overexpressing RCN4 had significantly increased branching, and initiated development of ~7× more spikelets than wild type. Our results reveal a role for OsMADS5 in panicle development, and show that OsMADS5 and OsMADS34 play similar functions in limiting branching and promoting the transition to spikelet meristem identity, in part by repressing RCN4 expression. These findings provide new insights to better understand the molecular regulation of rice inflorescence architecture.
dc.description.statementofresponsibilityWanwan Zhu, Liu Yang, Di Wu, Qingcai Meng, Xiao Deng, Guoqiang Huang, Jiao Zhang, Xiaofei Chen, Cristina Ferrándiz, Wanqi Liang, Ludovico Dreni, and Dabing Zhang
dc.identifier.citationNew Phytologist, 2022; 233(4):1682-1700
dc.identifier.doi10.1111/nph.17855
dc.identifier.issn0028-646X
dc.identifier.issn1469-8137
dc.identifier.orcidZhang, D. [0000-0003-3181-9812]
dc.identifier.urihttps://hdl.handle.net/2440/133827
dc.language.isoen
dc.publisherWiley
dc.relation.granthttp://purl.org/au-research/grants/arc/DP170103352
dc.rights© 2021 The Authors. New Phytologist © 2021 New Phytologist Foundation. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
dc.source.urihttps://doi.org/10.1111/nph.17855
dc.subjectInflorescence architecture and branching; MADS-box; meristem; OsMADS34; OsMADS5; RCN4, rice (Oryza sativa); TERMINAL FLOWER1
dc.titleRice SEPALLATA genes OsMADS5 and OsMADS34 cooperate to limit inflorescence branching by repressing the TERMINAL FLOWER1-like gene RCN4
dc.typeJournal article
pubs.publication-statusPublished

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