Lymphokines, including interleukin-2, alter gonadotropin-stimulated progesterone production and proliferation of human granulosa-luteal cells in vitro

Simple item page
dc.contributor.authorWang, L.
dc.contributor.authorRobertson, S.A.
dc.contributor.authorSeamark, R.F.
dc.contributor.authorNorman, R.J.
dc.date.issued1991
dc.description.abstractThe effects of human interleukin-1 (IL-1) and IL-2 on human granulosa-luteal cell progesterone production were examined with or without hCG stimulation in vitro. Human granulosa-luteal cells were recovered from follicular fluid obtained from women undergoing in vitro fertilization procedures and cultured for up to 7 days before supernatant progesterone level measurement. Lymphokine-rich conditioned medium was prepared from mitogen-stimulated human peripheral blood leukocytes (HPL-CM). The influence of HPL-CM on both granulosa-luteal cell progesterone production and cell growth was inhibitory. In contrast, supernatants of the IL-2-producing cell line MLA-144 (MLA-CM) stimulated both basal progesterone secretion and cell proliferation. Human recombinant IL-2 (from 0.1-100 IU) alone did not change progesterone levels, compared to control values, after 24 h of cell culture. However, 1, 10, and 100 IU IL-2 significantly inhibited progesterone secretion from cells stimulated by 5 IU hCG (P less than 0.01). The enhanced progesterone levels stimulated by forskolin were also significantly inhibited by 10 IU IL-2 (P = 0.01). This effect was not mediated through decreased cAMP, since the forskolin-enhanced cAMP level was not influenced by IL-2, IL-1, with or without hCG, did not show any effect on progesterone production during either 24 or 48 h of cell culture. It is concluded that 1) human recombinant IL-2 significantly inhibits progesterone production stimulated by hCG in human granulosa-luteal cells; 2) IL-2 also had a marked inhibitory effect on forskolin-induced progesterone release, but did not influence the increased cAMP level stimulated by forskolin; 3) the inhibitory influence of IL-2 on progesterone synthesis may be down-stream in the signal transduction pathway from cAMP activation; and 4) HPL-CM and MLA-CM produced inhibitory and stimulatory effects, respectively, on both basal and hCG-stimulated progesterone levels as well as on granulosa-luteal cell proliferation. These activities cannot be completely attributed to IL-2, and other mediators of leukocyte origin may, therefore, exist.
dc.description.statementofresponsibilityLingjia Wang, Sarah Robertson, Robert F. Seamark, and Robert J. Norman
dc.identifier.citationJournal of Clinical Endocrinology and Metabolism, 1991; 72(4):824-831
dc.identifier.doi10.1210/jcem-72-4-824
dc.identifier.issn0021-972X
dc.identifier.issn1945-7197
dc.identifier.orcidRobertson, S.A. [0000-0002-9967-0084]
dc.identifier.orcidNorman, R.J. [0000-0002-3118-3896]
dc.identifier.urihttp://hdl.handle.net/2440/85258
dc.language.isoen
dc.publisherEndocrine Society
dc.rightsCopyright © 1991 by The Endocrine Society
dc.source.urihttps://doi.org/10.1210/jcem-72-4-824
dc.subjectCorpus Luteum
dc.subjectGranulosa Cells
dc.subjectCells, Cultured
dc.subjectHumans
dc.subjectProgesterone
dc.subjectChorionic Gonadotropin
dc.subjectInterleukin-1
dc.subjectInterleukin-2
dc.subjectLymphokines
dc.subjectCulture Media
dc.subjectCell Division
dc.subjectFemale
dc.subjectColforsin
dc.titleLymphokines, including interleukin-2, alter gonadotropin-stimulated progesterone production and proliferation of human granulosa-luteal cells in vitro
dc.typeJournal article
pubs.publication-statusPublished

Files

Collections

Obstetrics and Gynaecology publications