Scrutinizing calcium flux oscillations in T lymphocytes to deduce the strength of stimulus
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Date
2015
Authors
Christo, S.
Diener, K.
Nordon, R.
Brown, M.
Griesser, H.
Vasilev, K.
Christo, F.
Hayball, J.
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Scientific Reports, 2015; 5(article no. 7760):7760-1-7760-8
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Susan N. Christo, Kerrilyn R. Diener, Robert E. Nordon, Michael P. Brown, Hans J. Griesser, Krasimir Vasilev, Farid C. Christo, John D. Hayball
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Abstract
The capture and activation of individual T cells on functionalised surfaces enables real-time analyses of the magnitude and rhythm of intracellular calcium release. Application of Haarlet transformations generate a calcium flux 'threshold', with the frequency of the 'threshold crossings' correlating with the strength of the original T cell stimulus. These findings represent a new method to evaluate graduations in T cell activation in real time, and at a single-cell level.
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