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Microstructured optical fiber-based biosensors: reversible and nanoliter-scale measurement of zinc ions

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dc.contributor.authorHeng, S.
dc.contributor.authorMcDevitt, C.A.
dc.contributor.authorKostecki, R.
dc.contributor.authorMorey, J.R.
dc.contributor.authorEijkelkamp, B.A.
dc.contributor.authorEbendorff-Heidepriem, H.
dc.contributor.authorMonro, T.M.
dc.contributor.authorAbell, A.D.
dc.date.issued2016
dc.descriptionPublished: May 6, 2016 Data source: Supporting information, http://pubs.acs.org/doi/abs/10.1021/acsami.6b03565
dc.description.abstractSensing platforms that allow rapid and efficient detection of metal ions would have applications in disease diagnosis and study, as well as environmental sensing. Here, we report the first microstructured optical fiber-based biosensor for the reversible and nanoliter-scale measurement of metal ions. Specifically, a photoswitchable spiropyran Zn(2+) sensor is incorporated within the microenvironment of a liposome attached to microstructured optical fibers (exposed-core and suspended-core microstructured optical fibers). Both fiber-based platforms retains high selectivity of ion binding associated with a small molecule sensor, while also allowing nanoliter volume sampling and on/off switching. We have demonstrated that multiple measurements can be made on a single sample without the need to change the sensor. The ability of the new sensing platform to sense Zn(2+) in pleural lavage and nasopharynx of mice was compared to that of established ion sensing methodologies such as inductively coupled plasma mass spectrometry (ICP-MS) and a commercially available fluorophore (Fluozin-3), where the optical-fiber-based sensor provides a significant advantage in that it allows the use of nanoliter (nL) sampling when compared to ICP-MS (mL) and FluoZin-3 (μL). This work paves the way to a generic approach for developing surface-based ion sensors using a range of sensor molecules, which can be attached to a surface without the need for its chemical modification and presents an opportunity for the development of new and highly specific ion sensors for real time sensing applications.
dc.description.statementofresponsibilitySabrina Heng, Christopher A. McDevitt, Roman Kostecki, Jacqueline R. Morey, Bart A. Eijkelkamp, Heike Ebendorff-Heidepriem, Tanya M. Monro, and Andrew D. Abell
dc.identifier.citationACS applied materials & interfaces, 2016; 8(20):12727-12732
dc.identifier.doi10.1021/acsami.6b03565
dc.identifier.issn1944-8244
dc.identifier.issn1944-8252
dc.identifier.orcidMcDevitt, C.A. [0000-0003-1596-4841]
dc.identifier.orcidKostecki, R. [0000-0001-5822-8401]
dc.identifier.orcidEijkelkamp, B.A. [0000-0003-0179-8977]
dc.identifier.orcidEbendorff-Heidepriem, H. [0000-0002-4877-7770]
dc.identifier.orcidAbell, A.D. [0000-0002-0604-2629]
dc.identifier.urihttp://hdl.handle.net/2440/99711
dc.language.isoen
dc.publisherAmerican Chemical Society
dc.relation.granthttp://purl.org/au-research/grants/arc/CE140100003
dc.relation.granthttp://purl.org/au-research/grants/arc/DP150101856
dc.rights© 2016 American Chemical Society
dc.source.urihttp://dx.doi.org/10.1021/acsami.6b03565
dc.subjectbiosensor
dc.subjectliposome
dc.subjectmicrostructured optical fiber
dc.subjectnanoscale
dc.subjectphotoswitch
dc.subjectzinc
dc.titleMicrostructured optical fiber-based biosensors: reversible and nanoliter-scale measurement of zinc ions
dc.typeJournal article
pubs.publication-statusPublished

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