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A rice glutamyl-tRNA synthetase modulates early anther cell division and patterning

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dc.contributor.authorYang, X.
dc.contributor.authorLi, G.
dc.contributor.authorTian, Y.
dc.contributor.authorSong, Y.
dc.contributor.authorLiang, W.
dc.contributor.authorZhang, D.
dc.date.issued2018
dc.description.abstractAminoacyl-tRNA synthetases (aaRSs) play a housekeeping role in cellular protein synthesis, but little is understood about how these aaRSs are involved in organ development. Here we report that, in the model crop rice (Oryza sativa), a glutamyl-tRNA synthetase (OsERS1) maintains proper somatic cell organization and limits the over-proliferation of male germinal cells during early anther development. The expression of OsERS1 is specifically detectable in meristematic layer 2-derived (L2-d) cells of the early anther, and osers1 exhibits over-proliferation and disorganization of L2-d cells, producing fused lobes and extra germinal cells in early anthers. The conserved biochemical function of OsERS1 in ligating glutamate to tRNAGlu, is enhanced by its cofactor OsARC (aminoacyl-tRNA synthetase co-factor). Further, metabolomics profiling reveals that OsERS1 is an important node for multiple metabolic pathways, indicated by the accumulation of amino acids and TCA components in osers1 anthers. Notably, the anther defects of osers1 are causally associated with the abnormal accumulation of H2O2, which can reconstitute the osers1 phenotype when applied to wild type. Collectively, this finding represents the first example on how aaRSs affect male organ development in plants, likely through protein synthesis, metabolic homeostasis and redox status.
dc.description.statementofresponsibilityXiujuan Yang, Gang Li, Yuesheng Tian, Yu Song, Wanqi Liang, Dabing Zhang
dc.identifier.citationPlant Physiology, 2018; 177(2):728-744
dc.identifier.doi10.1104/pp.18.00110
dc.identifier.issn0032-0889
dc.identifier.issn1532-2548
dc.identifier.orcidYang, X. [0000-0001-9340-3551]
dc.identifier.orcidZhang, D. [0000-0003-3181-9812]
dc.identifier.urihttp://hdl.handle.net/2440/116226
dc.language.isoen
dc.publisherAmerican Society of Plant Biologists
dc.rights© 2018 American Society of Plant Biologists. All rights reserved.
dc.source.urihttps://doi.org/10.1104/pp.18.00110
dc.subjectPlants, Genetically Modified
dc.subjectFlowers
dc.subjectMeristem
dc.subjectGlutamate-tRNA Ligase
dc.subjectGlutamic Acid
dc.subjectPlant Proteins
dc.subjectCell Division
dc.subjectGene Expression Regulation, Plant
dc.subjectMutation
dc.subjectOryza
dc.titleA rice glutamyl-tRNA synthetase modulates early anther cell division and patterning
dc.typeJournal article
pubs.publication-statusPublished

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