Mitogenic whey extract stimulates wound repair activity in vitro and promotes healing of rat incisional wounds
Date
2000
Authors
Rayner, T.
Cowin, A.
Gray, J.
Cooter, R.
Harries, R.
Regester, G.
Smithers, G.
Goddard, C.
Belford, D.
Editors
Advisors
Journal Title
Journal ISSN
Volume Title
Type:
Journal article
Citation
American Journal of Physiology. Regulatory Integrative and Comparative Physiology, 2000; 278(6 47-6):R1651-R1660
Statement of Responsibility
Timothy E. Rayner, Allison J. Cowin, J. Gray Robertson, Rodney D. Cooter, Richard C. Harries, Geoffrey O. Regester, Geoffrey W. Smithers, Chris Goddard, and David A. Belford
Conference Name
Abstract
The ability of single growth factors to promote healing of normal and compromised wounds has been well described, but wound healing is a process requiring the coordinated action of multiple growth factors. Only the synergistic effect on wound healing of combinations containing at most two individual growth factors has been reported. We sought to assess the ability of a novel milk-derived growth factor-enriched preparation ¿mitogenic bovine whey extract (MBWE), which contains six known growth factors, to promote repair processes in organotypic in vitro models and incisional wounds in vivo. MBWE stimulated the contraction of fibroblast-populated collagen lattices in a dose-dependent fashion and promoted the closure of excisional wounds in embryonic day 17 fetal rat skin. Application of MBWE increased incisional wound strength in normal animals on days 3, 5, 7, and 10 and reversed the decrease in wound strength observed following steroid treatment. Wound histology showed increased fibroblast numbers in wounds from normal and steroid-compromised animals. These data suggest the mixture of factors present in bovine milk exerts a direct action on the cells of cutaneous wound repair to enhance both normal and compromised healing.