Antibacterial spectrum and cytotoxic activities of serrulatane compounds from the Australian medicinal plant Eremophila neglecta

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dc.contributor.authorAnakok, O.
dc.contributor.authorNdi, C.
dc.contributor.authorBarton, M.
dc.contributor.authorGriesser, H.
dc.contributor.authorSemple, S.
dc.date.issued2012
dc.description.abstractAIMS: To determine the antibacterial spectrum and cytotoxic activities of serrulatane compounds from the Australian plant Eremophila neglecta. METHODS AND RESULTS: Antimicrobial activities of serrulatane compounds 8,19-dihydroxyserrulat-14-ene (1) and 8-hydroxyserrulat-14-en-19-oic acid (2) were tested against Gram-negative and Gram-positive bacteria including human and veterinary pathogens and some multidrug-resistant isolates. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations (MBCs) of the compounds were determined by broth microdilution assay. Both compounds exhibited antibacterial activity against all Gram-positive test strains. They showed antimycobacterial activity against isolates of Mycobacterium fortuitum and Mycobacterium chelonae. Of the five Gram-negative bacteria tested, only Moraxella catarrhalis showed susceptibility to the compounds. Cytotoxic activities were tested in the Vero cell line. Compound 1 showed more activity than 2 in both antibacterial and cytotoxicity assays with cytotoxicity at concentrations similar to the MBC. CONCLUSIONS: Serrulatane compounds showed significant activity against medically important bacteria, with 1 exhibiting stronger antibacterial activity. However, they also displayed toxicity to mammalian cells. SIGNIFICANCE AND IMPACT OF THE STUDY: Serrulatanes are of interest as novel antibacterial compounds for use in biomedical applications; this study reports data obtained with a range of bacterial strains and mammalian cells, essential for assessing the capabilities and limitations of potential applicability of these compounds.
dc.description.statementofresponsibilityO.F. Anakok, C.P. Ndi, M.D. Barton, H.J. Griesser, and S.J. Semple
dc.identifier.citationJournal of Applied Microbiology, 2012; 112(1):197-204
dc.identifier.doi10.1111/j.1365-2672.2011.05174.x
dc.identifier.issn1364-5072
dc.identifier.issn1365-2672
dc.identifier.urihttp://hdl.handle.net/2440/91125
dc.language.isoen
dc.publisherWiley
dc.rights© 2011 The Authors
dc.source.urihttps://doi.org/10.1111/j.1365-2672.2011.05174.x
dc.subjectantimicrobial activity; Australian medicinal plants; cytotoxicity; Eremophila neglecta; Myoporaceae; serrulatane diterpenoids
dc.titleAntibacterial spectrum and cytotoxic activities of serrulatane compounds from the Australian medicinal plant Eremophila neglecta
dc.typeJournal article
pubs.publication-statusPublished

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