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Functional differences in transport properties of natural HKT1;1 variants influence shoot Na⁺ exclusion in grapevine rootstocks

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dc.contributor.authorHenderson, S.
dc.contributor.authorDunlevy, J.
dc.contributor.authorWu, Y.
dc.contributor.authorBlackmore, D.
dc.contributor.authorWalker, R.
dc.contributor.authorEdwards, E.
dc.contributor.authorGilliham, M.
dc.contributor.authorWalker, A.
dc.date.issued2018
dc.description.abstractUnder salinity, Vitis spp. rootstocks can mediate salt (NaCl) exclusion from grafted V. vinifera scions enabling higher grapevine yields and production of superior wines with lower salt content. Until now, the genetic and mechanistic elements controlling sodium (Na(+) ) exclusion in grapevine were unknown. Using a cross between two Vitis interspecific hybrid rootstocks, we mapped a dominant quantitative trait locus (QTL) associated with leaf Na(+) exclusion (NaE) under salinity stress. The NaE locus encodes six high-affinity potassium transporters (HKT). Transcript profiling and functional characterization in heterologous systems identified VisHKT1;1 as the best candidate gene for controlling leaf Na(+) exclusion. We characterized four proteins encoded by unique VisHKT1;1 alleles from the parents, and revealed that the dominant HKT variants exhibit greater Na(+) conductance with less rectification than the recessive variants. Mutagenesis of VisHKT1;1 and TaHKT1.5-D from bread wheat, demonstrated that charged amino acid residues in the eighth predicted transmembrane domain of HKT proteins reduces inward Na(+) conductance, and causes inward rectification of Na(+) transport. The origin of the recessive VisHKT1;1 alleles was traced to V. champinii and V. rupestris. We propose that the genetic and functional data presented here will assist with breeding Na(+) -tolerant grapevine rootstocks.
dc.description.statementofresponsibilitySam W. Henderson, Jake D. Dunlevy, Yue Wu, Deidre H. Blackmore, Rob R. Walker, Everard J. Edwards, Matthew Gilliham, Amanda R. Walker
dc.identifier.citationNew Phytologist, 2018; 217(3):1113-1127
dc.identifier.doi10.1111/nph.14888
dc.identifier.issn0028-646X
dc.identifier.issn1469-8137
dc.identifier.orcidHenderson, S. [0000-0003-3019-1891]
dc.identifier.orcidWu, Y. [0000-0001-8994-1576]
dc.identifier.orcidWalker, R. [0000-0002-1409-7937]
dc.identifier.orcidGilliham, M. [0000-0003-0666-3078]
dc.identifier.urihttp://hdl.handle.net/2440/111386
dc.language.isoen
dc.publisherNew Phytologist Trust
dc.relation.granthttp://purl.org/au-research/grants/arc/CE140100008
dc.relation.granthttp://purl.org/au-research/grants/arc/FT130100709
dc.rights© 2017 The Authors. New Phytologist © 2017 New Phytologist Trust
dc.source.urihttps://doi.org/10.1111/nph.14888
dc.subject140 Ruggeri
dc.subjectK51-40
dc.subjectNorth American rootstocks
dc.subjectXenopus laevis oocytes
dc.subjecthybrids
dc.subjectsalinity
dc.subjectsite-directed mutagenesis
dc.subjectyeast
dc.titleFunctional differences in transport properties of natural HKT1;1 variants influence shoot Na⁺ exclusion in grapevine rootstocks
dc.title.alternativeFunctional differences in transport properties of natural HKT1;1 variants influence shoot Na(+) exclusion in grapevine rootstocks
dc.typeJournal article
pubs.publication-statusPublished

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